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Karyotypic relationships in Asiatic asses (kulan and kiang) as defined using horse chromosome arm-specific and region-specific probes

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Abstract

Cross-species chromosome painting has been applied to most of the species making up the numerically small family Equidae. However, comparative mapping data were still lacking in Asiatic asses kulan (Equus hemionus kulan) and kiang (E. kiang). The set of horse arm-specific probes generated by laser microdissection was hybridized onto kulan (E. hemionus kulan) and kiang (E. kiang) chromosomes in order to establish a genome-wide chromosomal correspondence between these Asiatic asses and the horse. Moreover, region-specific probes were generated to determine fusion configuration and orientation of conserved syntenic blocks. The kulan karyotype (2n = 54) was ascertained to be almost identical to the previously investigated karyotype of onager E. h. onager (2n = 56). The only difference is in fusion/fission of chromosomes homologous to horse 2q/3q, which are involved in chromosome number polymorphism in many Equidae species. E. kiang karyotype differs from the karyotype of E. hemionus by two additional fusions 8q/15 and 7/25. Chromosomes equivalent to 2q and 3q are not fused in kiang individuals with 2n = 52. Several discrepancies in centromere positions among kulan, kiang and horse chromosomes have been described. Most of the chromosome fusions in Asiatic asses are of centromere–centromere type. Comparative chromosome painting in kiang completed the efforts to establish chromosomal homologies in all representatives of the family Equidae. Application of region-specific probes allows refinement comparative maps of Asiatic asses.

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Abbreviations

2n:

diploid number of chromosomes

BAC:

bacterial artificial chromosome

EAS:

Equus asinus

ECA:

Equus caballus

EHK:

Equus hemionus kulan

EKI:

Equus kiang

EZH:

Equus zebra hartmannae

pd:

distal part of the p-arm

pp:

proximal part of the p-arm

qd:

distal part of the q-arm

qp:

proximal part of the q-arm

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Acknowledgements

The work was supported by the Grant Agency of the Czech Republic, project 523/09/1972 and by the Grant Agency of the Ministry of Agriculture of the Czech Republic, project MZE 0002716202.

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Authors and Affiliations

  1. Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic

    Petra Musilova, Svatava Kubickova & Jiri Rubes

  2. Faculty of Veterinary Medicine, Institute of Animal Genetics, Brno, Czech Republic

    Petr Horin

  3. The Prague Zoological Garden, Prague, Czech Republic

    Roman Vodička

Authors
  1. Petra Musilova
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  2. Svatava Kubickova
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  3. Petr Horin
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  4. Roman Vodička
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  5. Jiri Rubes
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Correspondence to Petra Musilova.

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Responsible Editor: Fengtang Yang.

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Musilova, P., Kubickova, S., Horin, P. et al. Karyotypic relationships in Asiatic asses (kulan and kiang) as defined using horse chromosome arm-specific and region-specific probes. Chromosome Res 17, 783–790 (2009). https://doi.org/10.1007/s10577-009-9069-3

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  • DOI: https://doi.org/10.1007/s10577-009-9069-3

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