Abstract
Subarachnoid hemorrhage (SAH) is a hemorrhagic cerebrovascular disease with an extremely poor prognosis. The molecular mechanism and biomarkers involved in neurological outcome after SAH still need to be explored. This study assessed the microRNA expression characteristics of SAH patients with different neurological outcomes by meta-analysis. Public databases were searched from database inception until December 2022. The study reported that microRNA expression data in SAH patients with different neurological outcomes were included in the analysis. The differential expression of miRNAs was evaluated by meta-analysis. Overrepresentation analysis was performed for the targeted genes of significant miRNAs. The XGBoost algorithm was used to assess the predictive ability for neurological outcomes with clinical characteristics and significantly expressed miRNAs. Seven studies were finally included in the meta-analysis. The results showed that the levels of miR-152-3p (SMD: − 0.230; 95% CI − 0.389, − 0.070; padj = 0.041), miR-221-3p (SMD: − 0.286; 95% CI − 0.446, − 0.127; padj = 0.007), and miR-34a-5p (SMD: − 0.227; 95% CI − 0.386, − 0.067; padj = 0.041) were significantly lower in SAH patients with good neurological outcomes than in those with poor neurological outcomes. The PI3K-AKT signaling pathway may have an important role in neurological recovery after SAH. Based on the XGBoost algorithm, the neurological outcome could be accurately predicted with clinical characteristics plus the three miRNAs. The expression levels of miR-152-3p, miR-221-3p, and miR-34a-5p were significantly lower in patients with good neurological outcomes than in those with poor outcomes. These miRNAs can serve as potential predictive biomarkers for neurological outcomes.
Graphical Abstract
The molecular mechanism and biomarkers involved in neurological outcome after SAH still need to be explored. Our study analyzed microRNA expression characteristics of SAH patients with different neurological outcomes by meta-analysis. After analyze studies reporting the microRNA expression data in SAH patients with different neurological outcomes, results show that the levels of miR-152-3p, miR-221-3p, and miR-34a-5p were significantly lower in SAH patients with good neurological outcomes than in those with poor neurological outcomes. The PI3K-AKT signaling pathway may have an important role in neurological recovery after SAH. Based on the XGBoost algorithm, the neurological outcome could be accurately predicted with clinical characteristics plus the three miRNAs.
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Data Availability
The datasets generated during and/or analysed during the current study are not publicly available but are available from the corresponding author on reasonable request.
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This work was supported by Appropriate Technology Extension Project of the Health and Health of the Autonomous Region in 2021(Clinician-led application and promotion of bedside intensive ultrasound technology) [SYTG-202172].
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by AA, BL and YC. The first draft of the manuscript was written by ZY and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Li, J., Liu, W., Anniwaer, A. et al. The Role of MicroRNAs in Predicting the Neurological Outcome of Patients with Subarachnoid Hemorrhage: A Meta-analysis. Cell Mol Neurobiol 43, 2883–2893 (2023). https://doi.org/10.1007/s10571-023-01327-7
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DOI: https://doi.org/10.1007/s10571-023-01327-7