Abstract
To reveal the dynamic process of cellulose biodegradation and explore more potential cellulases, a microbiota (FPDM) with cellulose-degrading ability was cultivated, and different stages of filter paper degradation were compared. Ion chromatography and comparative metagenomic sequencing revealed that the diversity of FPDM enhanced as the hydrolysate length diversity increased. Sporocytophaga and Cohnella dynamically dominated the synergistic degradation of cellulose in early-intermediate and intermediate-final periods, respectively. Moreover, 363 declining shifting and 231 progressive shifting unannotated genes were speculated to participate in the catabolism of cellulose to cellodextrin/cello-oligosaccharide and to cellobiose, respectively. Based on the dynamic changes in hydrolysates, community structure and gene abundance, a dynamic cellulose-degrading pathway of FPDM was predicted. Our work should provide a new perspective for subsequent identification of key cellulolytic strains and enzymes and clarification of the mechanism of cellulose biodegradation.
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Data availability
The metagenomic reads generated in this study have been submitted to NCBI Sequence Read Archive (SRA) under the accession number SRP255666. A previous version of this paper was published as preprint in Research Square (https://doi.org/10.21203/rs.3.rs-22654/v1) (Yang et al. 2020).
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FY, XL, and XC were supported by grants from the National Natural Sciences Foundation of China (31671796, 31771907, and 31801469), and the Liaoning BaiQianWan Talents Program is also gratefully acknowledged.
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This work was supported by the National Natural Sciences Foundation of China (31671796, 31771907, and 31801469), and the Liaoning BaiQianWan Talents Program is also gratefully acknowledged.
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MY: designed the strategy for metagenomic analysis, developed the scripts and wrote the manuscript. JZ: contributed with reagents, materials, and analyses, performed the experiments, and wrote the manuscript. YY, XC: contributed with reagents, materials, and performed the experiments. FY, XL: drafted, wrote and revised the manuscript. All authors agreed with the submitted version of the paper. All authors read and approved the final manuscript.
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Yang, M., Zhao, J., Yuan, Y. et al. Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota. Cellulose 28, 2105–2123 (2021). https://doi.org/10.1007/s10570-020-03671-z
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DOI: https://doi.org/10.1007/s10570-020-03671-z