Abstract
We report the first example of enzymatic synthesis of lutein dipalmitate in organic solvents by using a commercial immobilized Candida antarctica lipase B (Novozyme 435) as the catalyst. At the optimized condition, by using 20 mg/mL of lutein, 100 mg/mL of vinyl palmitate as the substrates, and 20 mg/mL of Novozyme 435 as the catalyst, the enzymatic synthesis in toluene achieved a yield of lutein dipalmitate of 83 % after 8 h reaction at 60 °C. The stability of the enzyme was demonstrated by over 80 % conversions for ten batches.
Graphical Abstract
A commercial immobilized Candida antarctica lipase B (Novozyme 435) was applied to catalyze the transerterification reaction between lutein and vinyl palmitate, achieving a yield of lutein dipalmitate of 83 % after 8 h reaction at 60 °C. The enzymatic synthesis of lutein dipalmitate received more than 80 % conversion for each batch during the 10 cycles of reuse.
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Acknowledgments
This work was supported by the National High Technology Research and Development Program (“863” Program) of China under the Grant number of 2014AA020507, the National Natural Science Foundation of China under the Grant numbers of 21036003 and 21206082, Tsinghua University Initiative Scientific Research Program under the Grant number of 20131089191.
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Wang, R., Hou, M., Zhang, Y. et al. Enzymatic Synthesis of Lutein Dipalmitate in Organic Solvents. Catal Lett 145, 995–999 (2015). https://doi.org/10.1007/s10562-015-1493-8
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DOI: https://doi.org/10.1007/s10562-015-1493-8