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Enhanced aptasensor performance for targeted HER2 breast cancer detection by using screen-printed electrodes modified with Au nanoparticles

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Abstract

The development of an Aptamer based biosensor for the selective detection of human epidermal growth factor receptor 2 (HER2) with high sensitivity and specificity was achieved. A screen-printed carbon electrode was used in the scope of this work. The HER2 Aptamer was immobilized via electrostatic adsorption on the surface of a screen-printed electrode, which was modified with Au Nanoparticles (~ 20 nm diameter) to support the Aptamer immobilization. The Aptasensor was extensively investigated using Cyclic voltammetry, Differential pulse voltammetry, Electrochemical impedance spectroscopy, Fourier transform infrared spectroscopy and Atomic force microscopy. The Aptasensor exhibits a fast response with a binding time of only 5 min and shows a log-linear response over a wide concentration range of 0.001—100 ng/mL. Moreover, it has high sensitivity and enhanced detection limit reaching 52.85 μA/ng/mL, and 0.001 ng/mL, respectively, with a relative standard deviation < 5%. The Aptasensor selectivity was studied by using different interfering substances, and the results demonstrate that the Aptasensor is efficient for the detection of HER2 with approximately 8% extent of the interference.

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Acknowledgements

This research was fully supported by the Royal Hashemite Courte under the Royal initiative for innovative projects in Nanotechnology.

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Authors and Affiliations

  1. Mechanical Engineering Department, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Tasneem Harahsheh

  2. Institute of Nanotechnology, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Yahia F. Makableh, Isra’ Rawashdeh & Mohamed Al-Fandi

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  1. Tasneem Harahsheh
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  2. Yahia F. Makableh
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  3. Isra’ Rawashdeh
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  4. Mohamed Al-Fandi
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Correspondence to Yahia F. Makableh.

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Harahsheh, T., Makableh, Y.F., Rawashdeh, I. et al. Enhanced aptasensor performance for targeted HER2 breast cancer detection by using screen-printed electrodes modified with Au nanoparticles. Biomed Microdevices 23, 46 (2021). https://doi.org/10.1007/s10544-021-00586-9

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