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Investigation of targeted biomolecules in a micro-fluxgate-based bio-sensing system

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Abstract

An investigation of targeted biomolecules was accomplished by combining a micro-fluxgate-based bio-sensing system and Dynabeads. The fluxgate sensor for biomolecule detection was fabricated by Micro Electro-Mechanical system technology, including thick photoresist lithography, electroplating and chemical wet etching. The magnetic core of the sensor was made of Fe-based amorphous ribbon core and three dimension solenoid coils were used as magnetic sensitive elements. The micro-fluxgate-based bio-sensing system was characterized firstly in different concentrations of Dynabeads, and a concentration as low as 100 ng/ml was detected with an external dc magnetic field in the range of 525 μT to 875 μT. Sandwich assays are performed using antibody-antigen pair combination of biotin-streptavidin on a separated Au film substrate surface with a self-assembled layer. Detection of Alpha Fetoprotein antigens with different concentrations was performed and a minimum detectable concentration of 1 pg/ml was achieved by the bio-sensing system. It is of considerable interest due to its potential application in the biomedical field based on known specific binding of target and labels.

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Acknowledgments

This work was supported by The Na-tional Natural Science Foundation of China (No. 61074168 and No. 61273065), National Science and Technology Support Program (2012BAK08B05), National Key Laboratory Research Fund (9140C790403110C7905), Natural Science Foundation of Shanghai (13ZR1420800) and the Analytical and Testing Center in Shanghai Jiao Tong University.

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Authors and Affiliations

  1. Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China

    Jian Lei, Chong Lei, Tao Wang, Zhen Yang & Yong Zhou

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  1. Jian Lei
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  2. Chong Lei
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  3. Tao Wang
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  4. Zhen Yang
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  5. Yong Zhou
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Correspondence to Chong Lei or Yong Zhou.

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Lei, J., Lei, C., Wang, T. et al. Investigation of targeted biomolecules in a micro-fluxgate-based bio-sensing system. Biomed Microdevices 16, 237–243 (2014). https://doi.org/10.1007/s10544-013-9827-z

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  • DOI: https://doi.org/10.1007/s10544-013-9827-z

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