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Chemiluminescence microfluidic system of gold nanoparticles enhanced luminol-silver nitrate for the determination of vitamin B12

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Abstract

A rapid and sensitive chemiluminescence (CL) system coupled with a microfluidic chip has been presented to determine vitamin B12 (VB12) based on the reaction of luminol and silver nitrate (AgNO3) in the presence of gold nanoparticles (AuNPs). A microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS) having four inlets and one outlet with a 200 μm wide, 250 μm deep, and 100 mm long microchannel. Ag+ was used as a chemiluminogenic oxidant in this CL reaction which oxidized luminol to produce strong CL signal in the presence of AuNPs. Luminol reacted with AgNO3 under the catalysis of AuNPs to produce luminol radicals which reacted with dissolved oxygen and emitted CL light. The proposed CL system was applied to determine the amount of VB12 in VB12 tablets and multivitamin. Under the optimum conditions, the CL intensity of the system was increased with the concentration of VB12 in the range of 0.25–100 ng mL−1 with the correlation coefficient of 0.9982. The limit of detection was found to be 0.04 ng mL−1 with the relative standard deviation of 1.56 % for five replicate determinations of 25 ng mL−1 of VB12. The CL reaction mechanism was demonstrated by UV-visible spectra and CL emission spectra.

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Acknowledgments

This work was supported by the Korea Research Council of Fundamental Science and Technology (KRCF) through Basic Research Project managed by the Korea Research Institute of Standards and Science (KRISS). This work was supported by the Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0093819).

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Authors and Affiliations

  1. Department of Chemistry, Kyungpook National University, Daegu, 702-701, South Korea

    Mohammad Kamruzzaman, Al-Mahmnur Alam, Kyung Min Kim & Sang Hak Lee

  2. Research Institute of Advanced Energy Technology, Kyungpook National University, Daegu, 702-701, South Korea

    Young Ho Kim

  3. Department of Applied Chemistry & Chemical Engineering, University of Dhaka, Dhaka, Bangladesh

    A. N. M. Hamidul Kabir

  4. School of Mechanical Engineering, Kyungpook National University, Daegu, 702-701, South Korea

    Gyu-Man Kim

  5. School of Mechanical Engineering, Yeungnam University, Gyeongbuk, 712-749, South Korea

    Trung Dung Dang

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  1. Mohammad Kamruzzaman
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  2. Al-Mahmnur Alam
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  3. Kyung Min Kim
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  4. Sang Hak Lee
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  5. Young Ho Kim
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Correspondence to Sang Hak Lee or Young Ho Kim.

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Kamruzzaman, M., Alam, AM., Kim, K.M. et al. Chemiluminescence microfluidic system of gold nanoparticles enhanced luminol-silver nitrate for the determination of vitamin B12. Biomed Microdevices 15, 195–202 (2013). https://doi.org/10.1007/s10544-012-9716-x

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