biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 60:261-268, 2016 | DOI: 10.1007/s10535-015-0578-y

Characterization of promoter activity of the ethylene receptor gene OgERS1 from Oncidesa in transgenic Arabidopsis

W.-F. Huang1, P.-L. Huang1,2, Y.-Y. Do1,*
1 Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei, Taiwan, ROC
2 Graduate Institute of Biotechnology, Chinese Culture University, Taipei, Taiwan, ROC

Physiological changes associated with senescence of flowers and abscission of floral parts in Oncidesa (formerly Oncidium) cv. Gower Ramsey are caused by a plant hormone ethylene which is produced by pollinia cap dislodgment during postharvest handling and transportation. The ethylene receptor gene OgERS1 of Oncidesa has been previously cloned and characterized. To analyze promoter activity of OgERS1, transgenic Arabidopsis thaliana plants were generated to express the ß-glucuronidase (GUS) reporter gene under the control of 5'-upstream sequence of OgERS1 from Oncidesa. The expression pattern of the OgERS1 promoter at the cellular level was investigated by analysis of GUS activity. This promoter can activate gene expression in both actively dividing young tissues and abscission-related aging tissues. Expression of GUS was detected in the shoot meristem uniquely in 10 to 30 d-old-plants and was found in flower buds, axillary buds, flower stems, and abscission layers during later development. In 2- to 3-week-old transgenic Arabidopsis, exogenous ethylene, glucose, lactose, and maltose enhanced promoter activity implying that crosstalk between sugar and an ethylene receptor may exist. However, indole-3-acetic acid, benzylaminopurine, abscisic acid, heat, wounding, salinity, drought, and flooding slightly suppressed promoter activity. These results demonstrate that the promoter of OgERS1 was developmentally and environmentally regulated, and imply a potential for application of this bi-functional promoter to increase branching or enhanced dwarfing.

Keywords: abscisic acid; abscission layer; actively dividing young tissue; benzylaminopurine; drought; flooding; heat; indole-3-acetic acid; methyl jasmonate; salinity; sugars; wounding
Subjects: gene promoter; ethylene receptor; transgenic plant; abscsic acid; abscission zone; benzyladenine; indoleacetic acid; methyl jasmonate; drought; flooding; salinity; wounding

Received: May 2, 2015; Revised: June 25, 2015; Accepted: July 27, 2015; Published: June 1, 2016  Show citation

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Huang, W.-F., Huang, P.-L., & Do, Y.-Y. (2016). Characterization of promoter activity of the ethylene receptor gene OgERS1 from Oncidesa in transgenic Arabidopsis. Biologia plantarum, 60(2), 261-268. doi: 10.1007/s10535-015-0578-y
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    References

    1. Abeles, F.B., Morgan, P.W., Saltveit, M.E.: Ethylene in Plant Biology. - Academic Press, San Diego 1992.
    2. Agarwal, G., Choudhary, D., Singh, V.P., Arora, A.: Role of ethylene receptors during senescence and ripening in horticultural crops. - Plant Signal Behav. 7: 827-846, 2012. Go to original source...
    3. Beaudoin, N., Serizet, C., Gosti, F., Giraudat, J.: Interactions between abscisic acid and ethylene signaling cascades. - Plant Cell 12: 1103-1116, 2000. Go to original source...
    4. Bechtold, N., Pelletier, G.: In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. - Methods mol. Biol. 82: 259-266, 1998. Go to original source...
    5. Brady, C.J.: Fruit ripening. - Annu. Rev. Plant Physiol. 38: 155-178, 1987. Go to original source...
    6. Butenko, M.A., Patterson, S.E., Grini, P.E., Stenvik, G.E., Amundsen, S.S., Mandal, A., Aalen, R.B.: INFLORESCENCE DEFICIENT IN ABSCISSION controls floral organ abscission in Arabidopsis and identifies a novel family of putative ligands in plants. - Plant Cell 15: 2296-2307, 2003. Go to original source...
    7. Dervinis, C., Clark, D.G., Barrett, J.E., Nell, T.A.: Effect of pollination and exogenous ethylene on accumulation of ETR1 homologue transcripts during flower petal abscission in geranium (Pelargonium × hortorum L.H. Bailey). - Plant mol. Biol. 42: 847-856, 2000. Go to original source...
    8. Gazzarrini, S., McCourt, P.: Genetic interactions between ABA, ethylene and sugar signaling pathways. - Curr. Opin. Plant Biol. 4: 387-391, 2001. Go to original source...
    9. Ghassemian, M., Nambara, E., Cutler, S., Kawaide, H., Kamiya, Y., McCourt, P.: Regulation of abscisic acid signaling by the ethylene response pathway in Arabidopsis. - Plant Cell 12: 1117-1126, 2000. Go to original source...
    10. Grefen, C., Städele, K., Ru¾icka, K., Obrdlik, P., Harter, K., Horák, J.: Subcellular localization and in vivo interactions of the Arabidopsis thaliana ethylene receptor family members. - Mol. Plant 1: 308-320, 2008. Go to original source...
    11. Guzman, P., Ecker, J.R.: Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. - Plant Cell 2: 513-523, 1990. Go to original source...
    12. Hua, J., Sakai, H., Nourizadeh, S., Chen, Q.G., Bleecker, A.B., Ecker, J.R., Meyerowitz, E.M.: EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis. - Plant Cell 10: 1321-1332, 1998. Go to original source...
    13. Huang, W.-F., Huang, P.-L., Do, Y.-Y.: Ethylene receptor transcript accumulation patterns during flower senescence in Oncidium 'Gower Ramsey' as affected by exogenous ethylene and pollinia cap dislodgment. - Postharvest Biol. Technol. 44: 87-94, 2007. Go to original source...
    14. Ito, M., Sato, T., Fukuda, H., Komamine, A.: Meristem-specific gene expression directed by the promoter of the S-phase-specific gene, cyc07, in transgenic Arabidopsis. - Plant mol. Biol. 24: 863-878, 1994. Go to original source...
    15. Jefferson, R.A., Kavanagh, T.A., Bevan, M.W.: GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. - EMBO J. 6: 3901-3907, 1987. Go to original source...
    16. Lashbrook, C.C., Tieman, D.M., Klee, H.J.: Differential regulation of the tomato ETR gene family throughout plant development. - Plant J. 15: 243-252, 1998. Go to original source...
    17. Lü, S., Gu, H., Yuan, X., Wang, X., Wu, A.M., Qu, L., Liu J.Y.: The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis. - Transgenic Res. 16: 177-191, 2007. Go to original source...
    18. Müller, R., Lind-Iversen, S., Stummann, B.M., Serek, M.: Expression of genes for ethylene biosynthetic enzymes and an ethylene receptor in senescing flowers of miniature potted roses. - J. hort. Sci. Biotechnol. 75: 12-18, 2000b. Go to original source...
    19. Müller, R., Stummann, B.M., Andersen, A.S., Serek, M.: Involvement of ABA in postharvest life of miniature potted roses. - Plant Growth Regul. 29: 143-150, 1999. Go to original source...
    20. Müller, R., Stummann, B.M., Serek, M.: Characterization of an ethylene receptor family with differential expression in rose (Rosa hybrida L.) flowers. - Plant Cell Rep. 19: 1232-1239, 2000a. Go to original source...
    21. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    22. Nichols, R.: The response of carnations (Dianthus caryophyllus) to ethylene. - J. hort. Sci. 43: 335-349, 1968. Go to original source...
    23. Osborne, D.J.: Ethylene. - In: Latham, D.S., Goodwin, P.B., Higgins, T.J.V. (ed.): Phytohormones and Related Compounds: a Comprehensive Treatise. P. 266. Elsevier, Amsterdam 1978.
    24. Osborne, D.J.: The ethylene regulation of cell growth in specific target tissues of plants. - In: Wareing, P.F. (ed.): Plant Growth Substances. P. 279. Academic Press, London 1984.
    25. Patterson, S.E., Bleecker, A.B.: Ethylene-dependent and independent processes associated with floral organ abscission in Arabidopsis. - Plant Physiol. 134: 194-203, 2004. Go to original source...
    26. Payton, S., Fray, R.G., Brown, S., Grierson, D.: Ethylene receptor expression is regulated during fruit ripening, flower senescence and abscission. - Plant mol. Biol. 31: 1227-1231, 1996. Go to original source...
    27. Rolland, F., Moore, B., Sheen, J.: Sugar sensing and signaling in plants. - Plant Cell 14: S185-S205, 2002. Go to original source...
    28. Shibuya, K., Nagata, M., Tanikawa, N., Yoshihito, T., Hashiba, T., Satoh, S.: Comparison of mRNA levels of three ethylene receptors in senescing flowers of carnation (Dianthus caryophyllus L.). - J. exp. Bot. 53: 399-406, 2002. Go to original source...
    29. Tanase, K., Ichimura, K.: Expression of ethylene receptors DI-ERS1-3 and DI-ERS2, and ethylene response during flower senescence in Delphinium. - J. Plant Physiol. 163: 1159-1166, 2006. Go to original source...
    30. Thongkum, M., Bhunchoth, A., Warin, N., Charchawankanphanich, O., Burns, P.: Cloning and expression of Ethylene Response Sensor 1 (Den-ERS1) gene of Dendrobium 'Pompadour' flower during development and senescence. - Thai J. agr. Sci. 42: 227-236, 2009.
    31. Wang, N.N., Shih, M.-C., Li, N.: The GUS reporter-aided analysis of the promoter activities of Arabidopsis ACC synthase genes AtACS4, AtACS5, and AtACS7 induced by hormones and stresses. - J. exp. Bot. 56: 909-920, 2005. Go to original source...
    32. Welchen, E., Chan, R.L., Gonzalez, D.H.: The promoter of the Arabidopsis nuclear gene COX5b-1, encoding subunit 5b of the mitochondrial cytochrome c oxidase, directs tissue-specific expression by a combination of positive and negative regulatory elements. - J. exp. Bot. 55: 1997-2004, 2004. Go to original source...
    33. Wilkinson, J.E., Twell, D., Lindsey, K.: Activities of CaMV 35S and nos promoters in pollen: implications for field release of transgenic plants. - J. exp. Bot. 48: 265-275, 1997. Go to original source...
    34. Xu, Y., Chang, P.-F. L., Liu, D., Narasimhan, M.L., Raghothama, K.G., Hasegawa, P.M., Bressan, R.A.: Plant defense genes are synergistically induced by ethylene and methyl jasmonate. - Plant Cell 6: 1077-1085, 1994. Go to original source...
    35. Xue, J., Li, Y., Tan, H., Yang, F., Ma, N., Gao, J.: Expression of ethylene biosynthetic and receptor genes in rose floral tissues during ethylene-enhanced flower opening. - J. exp. Bot. 59: 2161-2169, 2008. Go to original source...
    36. Yang, S.F., Hoffman, N.E.: Ethylene biosynthesis and its regulation in higher plants. - Annu. Rev. Plant Physiol. 35: 155-189, 1984. Go to original source...

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