biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:479-490, 2014 | DOI: 10.1007/s10535-014-0429-2

Molecular cloning and expression analysis of four turmeric MAP kinase genes in response to abiotic stresses and phytohormones

S. Nanda1, S. Nayak1, R. K. Joshi1,*
1 Centre of Biotechnology, Siksha O. Anusandhan University, Bhubaneswar, India

Plant mitogen activated protein kinase (MAPK) cascades comprise a complex network playing a major role in regulating extracellular stimuli as well as developmental processes. The present study involves cloning four MAPKs (ClMPK1, 3, 4 and 5) from Curcuma longa. All four ClMPKs have fully canonical motifs of MAPK and each is represented by a single copy in the turmeric genome. The analysis of exon-intron junctions revealed conserved nature of ClMPKs across different plant groups. The RT-qPCR analysis showed their expression in mature plant tissues. The transcript analysis using the RT-qPCR shows that the four ClMPKs were differentially regulated by cold, salinity, and drought stresses. ClMPK4 showed a significant upregulation in the presence of NaCl, polyethylene glycol, and mannitol. The time-course expression analysis revealed a marked accumulation of ClMPK1 and ClMPK4 transcripts after mechanical wounding or applications of abscisic acid, H2O2, methyl jasmonate, and salicylic acid. ClMPK5 showed a unique and pronounced expression in response to hexavalent chromium (CrVI).

Keywords: abscisic acid; ClMPKs; chromium; cold; Curcuma longa; methyl jasmonate; RT-qPCR; salinity; water stress
Subjects: MAP kinase; abscisic acid; temperature - low; salinity; methyl jasmonate; water stress; gene expression; amino acids; chromium; phylogenetic tree; salicylic acid; hydrogen peroxide; nitric oxide; turmeric

Received: August 1, 2013; Revised: February 5, 2014; Accepted: February 6, 2014; Published: September 1, 2014  Show citation

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Nanda, S., Nayak, S., & Joshi, R.K. (2014). Molecular cloning and expression analysis of four turmeric MAP kinase genes in response to abiotic stresses and phytohormones. Biologia plantarum, 58(3), 479-490. doi: 10.1007/s10535-014-0429-2
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