biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:389-394, 2012 | DOI: 10.1007/s10535-012-0105-3

Expression of the BBM gene during somatic embryogenesis of Arabidopsis thaliana

K. Kulinska-Lukaszek1,*, M. Tobojka1, A. Adamiok1, E. U. Kurczynska1
1 Laboratory of Cell Biology, University of Silesia, Katowice, Poland

The relationship between somatic embryogenesis (SE) and the expression of the BABY BOOM (BBM) gene was studied in cultured immature zygotic embryos (IZEs) using a transgenic line of Arabidopsis thaliana containing a BBMPro::GUS construct. Results showed spatio-temporal differences in BBM expression in explants during culture. BBM promoter activity was observed in freshly isolated IZEs except distal parts of cotyledons. At the beginning of culture, considerable increase of GUS staining intensity was observed in all parts of explants, which maintained at high level over next few days and coincide with cell divisions. Gradual decrease of GUS distribution in explants was observed at about the 5th day of culture. BBM promoter activity became largely restricted to dividing cells, then to developing somatic embryos, shoot-like structures and callus. In parts of explants not involved in morphogenesis BBM promoter activity was absent or hardly seen. Thus the in vitro expression of BBM coincides with cell proliferation and morphogenesis.

Keywords: GUS construct; immature zygotic embryos
Subjects: immature zygotic embryos; BBM gene; transgenic plants; gene construct; GUS expression; somatic embryogenesis

Received: February 5, 2011; Accepted: May 20, 2011; Published: June 1, 2012  Show citation

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Kulinska-Lukaszek, K., Tobojka, M., Adamiok, A., & Kurczynska, E.U. (2012). Expression of the BBM gene during somatic embryogenesis of Arabidopsis thaliana. Biologia plantarum, 56(2), 389-394. doi: 10.1007/s10535-012-0105-3
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