biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:323-326, 2011 | DOI: 10.1007/s10535-011-0046-2

Genetic variation within and among small isolated populations of Santalum album

K. G. Srikanta Dani1, P. Ravikumar1, R. Pravin Kumar1, A. Kush1,*
1 Vittal Mallya Scientific Research Foundation, Bangalore, India

A combination of directed amplification of minisatellite DNA (DAMD) and random amplification of polymorphic DNA (RAPD) primes were used to assess the genetic variation within and between three isolated populations of Indian sandalwood (Santalum album). Eleven primers used in this study amplified 65.99 % polymorphic bands. Analysis of molecular variance revealed a high genetic variation among these populations (ϕST = 0.549). There are indications of clonality within the existing Indian sandalwood populations which can be attributed to habitat fragmentation, isolation and vegetative reproduction.

Keywords: DAMD; genetic diversity; population fragmentation; RAPD; sandalwood

Received: May 10, 2009; Accepted: December 17, 2009; Published: June 1, 2011  Show citation

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Srikanta Dani, K.G., Ravikumar, P., Pravin Kumar, R., & Kush, A. (2011). Genetic variation within and among small isolated populations of Santalum album. Biologia plantarum55(2), 323-326. doi: 10.1007/s10535-011-0046-2
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References

  1. Aagaard, J.E.: RAPDs and allozymes exhibit similar levels of diversity and differentiation among populations and races of Douglas-fir. - Heredity 81: 69-78, 1998. Go to original source...
  2. Aguilar, R., Quesda, M., Ashworth, L., Herrerias-Diego, Y., Lobo, G.: Genetic consequences of habitat fragmentation in plant populations: susceptible signals in plant traits and methodological approaches. - Mol. Ecol. 17: 5177-5188, 2008. Go to original source...
  3. Amiri, R., Mesbah, M., Moghaddam, M., Bihamta, M.R., Mohammadi, S.A., Norouzi, P.: A new RAPD marker for beet necrotic yellow vein virus resistance gene in Beta vulgaris. - Biol. Plant. 53: 112-119, 2009. Go to original source...
  4. Bhattacharya, E., Ranade, S.A.: Molecular distinction amongst varieties of mulberry using RAPD and DAMD profiles. - BMC Plant Biol. 1: 3-5, 2001. Go to original source...
  5. Bottin, L., Verhaegen, D., Tassin, J., Olivieri, I., Valliant, A., Bouvet, J.M.: Genetic diversity and population structure of an insular tree, Santalum austrocaledonicum in new caledonian archipelago. - Mol. Ecol. 14: 1979-1989, 2005. Go to original source...
  6. Brand, J.E.: Genotypic variation in Santalum album. - Sandalwood Res. Newslett. 2: 2-4, 1994.
  7. Dice, L.R.: Measures of the amount of ecologic association between species. - Ecology 26: 297-302, 1945. Go to original source...
  8. Doyle, J.J., Doyle, J.L.: Isolation of plant DNA from fresh tissues. - Focus. 12: 13-15, 1995.
  9. Feria-Romero, I.A., Astudillo-de la Vega, H., Chavez-Soto, M.A., Rivera-Arce, E., López, M., Serrano, H., Lozoya, X. RAPD markers associated with quercetin accumulation in Psidium guajava. - Biol. Plant. 53: 125-128, 2009. Go to original source...
  10. Frankham, R.: Do island populations have less genetic variation than mainland populations? - Heredity 78: 311-327, 1997. Go to original source...
  11. Hochbaum, D.S., Pathria, A.: Path costs in evolutionary tree reconstruction. - J. Computat. Biol. 4: 163-176, 1997. Go to original source...
  12. Lacerda, D.R., Acedo, M.D.P., Lemnos Filho, J.P., Lovato, M.B.: Genetic diversity and structure of natural populations of Plathymenia reticulate (Mimosoideae), a tropical tree from Brazilian cerrado. - Mol. Ecol. 9: 1843-1853, 2001.
  13. Lhuillier, E., Butaud, J.F., Bouvet, J.M.: Extensive clonality and strong differentiation in the insular pacific tree Santalum insulare: implications for its conservation. - Ann. Bot. 98:1061-1072, 2006. Go to original source...
  14. Muir, K., Byrne, M., Barbour, E., Cox, M.C., Fox, J.E.D.: High levels of out-crossing in a family trial of Western Australian sandalwood (Santalum spicatum). - Silvae Genet. 56: 222-230, 2007. Go to original source...
  15. Nei, M., Li, W.H.: Mathematical model for studying genetic variation in terms of restriction endonucleases. - Proc. nat. Acad. Sci. USA 76: 5269-5273, 1979. Go to original source...
  16. Rao, M.N., Ganeshaiah, K.N., Shaanker, U.: Assessing threats and mapping sandal resources to identify genetic hot spots for in situ conservation in peninsular India. - Conserv. Genet. 8: 925-935, 2007. Go to original source...
  17. Shashidhara, G., Hema, M.V., Koshy, B., Farooki, A.A.: Assessment of genetic diversity and identification of core collection in sandalwood germplasm using RAPDs. - J. hort. Sci. Biotechol. 78: 528-536, 2003. Go to original source...
  18. Shetty, R.H.: Sandal, an exotic tree? - Indian Forest. 103: 359-367, 1977.
  19. Shrestha, M.K., Golan-Goldhrish, A., Ward, D.: Population genetic structure and conservation of isolated populations of Acacia raddiana in Negev desert. - Biol. Conserv. 108: 119-127, 2002. Go to original source...
  20. Stockwell, C.A.: Contemporary evolution meets conservation biology. - Trends Ecol. Evol. 18: 94-101, 2003. Go to original source...
  21. Tourmente, S., Lazreg, A., Lafleuriel, J., Tutois, S., Cuvillier, C., Espagnol, M.C., Picard, G.: Identification of new minisatellite loci in Arabidopsis thaliana. - J. exp. Bot. 49:21-25, 1998. Go to original source...
  22. Zhou, J., Bebeli, P.J., Somers, D.J., Gustafson, J.P.: Directed amplification of minisatellite region DNA in VNTR core sequences in the genus Oryza. - Theor. appl. Genet. 95:942-949, 1997. Go to original source...