biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:195-197, 2010 | DOI: 10.1007/s10535-010-0034-y

Concurrent occurrence of α-amylase inhibitor and stimulator in red kidney bean seed: physiological implications

H. Alizadeh1,2, D. W. M. Leung1,*, A. L. J. Cole1
1 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand
2 Department of Plant Protection, Aboureyhan Pardis, University of Tehran, Tehran, Iran

It is hypothesized that since protein α-amylase inhibitor (α-AI) and stimulator might be present together in red kidney bean (Phaseolus vulgaris L.) seeds, their in vitro interactions might influence their detection and quantification. Assay of α-AI using extracts from the embryonic axes revealed an unexpected finding in that the extracts stimulated rather than inhibited α-amylase activity. The cotyledon extracts exhibited inhibitory or enhancement effect on α-amylase activity depending on whether prior to the α-amylase assay they had been boiled for 10 min or not. Phytohemagglutinin (PHA-L in particular) is implicated in the present study as a stimulator of α-amylase activity co-extracted with α-AI from red kidney bean cotyledons. The importance of these findings is discussed in relation to the possible widespread occurrence of protein α-amylase stimulator in seeds and other plant parts.

Keywords: lectins; Phaseolus vulgaris; phytohemagglutinins
Subjects: amylase; Phaseolus vulgaris; red kidney bean

Received: March 20, 2009; Accepted: December 3, 2009; Published: March 1, 2010  Show citation

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Alizadeh, H., Leung, D.W.M., & Cole, A.L.J. (2010). Concurrent occurrence of α-amylase inhibitor and stimulator in red kidney bean seed: physiological implications. Biologia plantarum54(1), 195-197. doi: 10.1007/s10535-010-0034-y
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