biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:291-298, 2008 | DOI: 10.1007/s10535-008-0061-0

Stress-induced degradation of D1 protein and its photoprotection by DCPIP in isolated thylakoid membranes of barley leaf

D. M. Pandey1, U. -D. Yeo1
1 Faculty of Biological Science, Chonbuk National University, Jeonju, Republic of Korea

Effects of various stress treatments such as NaCl, hydrogen peroxide, hydroxyl free radical, and high irradiance (HI, 1 000 µmol m-2 s-1) on the photosystem (PS) 2 mediated electron transport rate and the degradation of D1 protein in the thylakoid membranes of barley were studied. The applied stresses caused significant reduction in the PS 2-mediated electron transport and a degradation of D1 protein that was highest during the HI-treatment. Presence of 2,6-dichlorophenol indophenol (DCPIP), which is an artificial electron acceptor from water, significantly minimizes the HI-induced deleterious effect on the PS 2-mediated electron transport rate, disarrangement of PS machinery, and degradation of the D1 protein. HI in the absence of an acceptor resulted in production of reactive oxygen species due to electron transfer to oxygen.

Keywords: DCPIP; electron transport rate; Hordeum vulgare; photoinhibition
Subjects: chloroplasts; electron transport rate; Hordeum vulgare; photoinhibition; proteins

Received: February 3, 2006; Accepted: December 6, 2006; Published: June 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Pandey, D.M., & Yeo, U.-D. (2008). Stress-induced degradation of D1 protein and its photoprotection by DCPIP in isolated thylakoid membranes of barley leaf. Biologia plantarum, 52(2), 291-298. doi: 10.1007/s10535-008-0061-0
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Allakhverdiev, S.I., Murata, N.: Environmental stress inhibits the synthesis de novo of proteins involved in the photodamage-repair cycle of photosystem II in Synechocystis sp. PCC 6803.-Biochim. biophys. Acta 1657: 23-32, 2004. Go to original source...
    2. Allakhverdiev, S.I., Sakamoto, A., Nishiyama, Y., Inaba, M., Murata, N.: Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.-Plant Physiol. 123: 1047-1056, 2000a. Go to original source...
    3. Allakhverdiev, S.I., Sakamoto, A., Nishiyama, Y., Murata, N.: Inactivation of photosystems I and II in response to osmotic stress in Synechococcus: contribution of water channels.-Plant Physiol. 122: 1201-1208, 2000b. Go to original source...
    4. Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of photosystem II. Inactivation, protein damage and turnover.-Biochim. biophys. Acta 1143: 113-134, 1993. Go to original source...
    5. Baena-Gonzalez, E., Aro, E.-M.: Biogenesis, assembly and turnover of photosystem II units.-Phil. Trans. biol. Sci. 357: 1451-1460, 2002. Go to original source...
    6. Bishnoi, S.K., Kumar, B., Rani, C., Datta, K.S., Kumari, P., Sheoran, I.S., Angrish, R.: Changes in protein profile of pigeon pea genotypes in response to NaCl and boron stress.-Biol. Plant. 50: 135-137, 2006. Go to original source...
    7. Bradley, R.L., Long, K.M., Frasch, W.D.: The involvement of photosystem II-generated H2O2 in photoinhibition.-FEBS Lett. 286: 209-213, 1991. Go to original source...
    8. Busogoro, J.P., Etamé, J.J., Lognay, G., Messiaen, J., Van Cutsem, P., Lepoivre, P.: Analysis of the mechanisms of action of Mycosphaerella fijiensis toxins during the development of black leaf streak disease.-In: Jain, S.M., Swennen, R. (ed.): Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Pp. 392. Science Publishers, Enfield 2004.
    9. Donahue, J.L., Okpodu, C.M., Cramer, C.L., Grabau, E.A., Alscher, R.G.: Responses of antioxidants to paraquat in pea leaves.-Plant Physiol. 113: 249-257, 1997. Go to original source...
    10. Enami, I., Kamino, K., Shen, J.-R., Satoh, K., Katoh, S.: Isolation and characterization of photosystem II complexes which lack light-harvesting chlorophyll a/b proteins but retain three extrinsic proteins related to oxygen evolution from spinach.-Biochim. biophys. Acta 977: 33-39, 1989. Go to original source...
    11. Fujii, T., Yokoyama, E., Inoue, K., Sakurai, H.: The sites of electron donation of photosystem I to methyl viologen.-Biochim. biophys. Acta 1015: 41-48, 1990. Go to original source...
    12. Halliwell, B., Gutteridge, J.M.C.: Oxygen toxicity, oxygen radicals, transition metals and disease.-Biochem. J. 219: 1-14, 1984. Go to original source...
    13. Henmi, T., Miyao, M., Yamamoto, Y.: Release and reactive-oxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition.-Plant Cell Physiol. 45: 243-250, 2004. Go to original source...
    14. Henmi, T., Yamasaki, H., Sakuma, S., Tomokawa, Y., Tamura, N., Shen, J.-R., Yamamoto, Y.: Dynamic interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts: Evidence from light-induced cross-linking of the proteins in the donor-side photoinhibition.-Plant Cell Physiol. 44: 451-456, 2003. Go to original source...
    15. Hernández, J.A., Olmos, E., Corpas, F.J., Sevilla, F., del Rio, L.A.: Salt-induced oxidative stress in chloroplasts of pea plants.-Plant Sci. 105: 151-167, 1995. Go to original source...
    16. Hofman, P., Haisel, D., Komenda, J., Vágner, M., Tichá, I., Schäafer, C., Čapková, V.: Impact of in vitro cultivation conditions on stress responses and changes in thylakoid membrane proteins and pigments of tobacco during ex vitro acclimation.-Biol. Plant. 45: 189-195, 2002. Go to original source...
    17. Hundal, T., Virgin, I., Styring, S., Andersson, B.: Changes in the organization of photosystem II following light-induced D1-protein degradation.-Biochim. biophys. Acta 1017: 235-241, 1990. Go to original source...
    18. Kashino, Y., Koike, H., Satoh, K.: An improved sodium dodecyl sulfate-polyacrylamide gel electrophoresis system for the analysis of membrane protein complexes.-Electrophoresis 22: 1004-1007, 2001. Go to original source...
    19. Liu, W.-J., Yuan, S., Zhang, N.-H., Lei, T., Duan, H.-G., Liang, H.-G., Lin H.-H.: Effect of water stress on photosystem 2 in two wheat cultivars.-Biol. Plant. 50: 597-602, 2006. Go to original source...
    20. Mano, J., Ohno, C., Domae, Y., Asada, K.: Chloroplastic ascorbate peroxidase is the primary target of methylviologen-induced photo-oxidative stress in spinach leaves: its relevance to monodehydroascorbate radical detected with in vivo ESR.-Biochim. biophys. Acta 1504: 275-287, 2001. Go to original source...
    21. Maslenkova, L., Gambarova, N., Zeinalov, Y.: NaCl-induced changes in oxygen evolving activity and thylakoid membrane patterns of barely plants. Adaptation to salinity.-Bulg. J. Plant Physiol. 21: 29-35, 1995.
    22. Mattoo, A.K., Hoffman-Falk, H., Marder, J.B., Edelman, M.: Regulation of protein metabolism: Coupling of photosynthetic electron transport to in vivo degradation of the rapidly metabolized 32-kilodalton protein of the chloroplast membranes.-Proc. nat. Acad. Sci. USA 81: 1380-1384, 1984. Go to original source...
    23. Miyao, M., Ikeuchi, M., Yamamoto, N., Ono, T.: Specific degradation of the D1 protein of photosystem II by treatment with hydrogen peroxide in darkness: Implications for the mechanism of degradation of the D1 protein under illumination.-Biochemistry 34: 10019-10026, 1995. Go to original source...
    24. Murata, N., Mohanty, P.S., Hayashi, H., Papageorgiou, G.: Glycinebetaine stabilizes the association of extrinsic proteins with the photosynthetic oxygen-evolving complex.-FEBS Lett. 296: 187-189, 1992. Go to original source...
    25. Muthuchelian, K., Bertamini, M., Nedunchezhian, N.: Iron deficiency induced changes on electron transport rate in Pisum sativum chloroplast.-Biol. Plant. 44: 313-316, 2001. Go to original source...
    26. Nishiyama, Y., Yamamoto, H., Allakhverdiev, S.I., Inaba, M., Yokota, A., Murata, N.: Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery.-EMBO J. 20: 5587-5594, 2001. Go to original source...
    27. Nováková, M., Matějová, E., Sofrová, D.: Cd+2 effect on photosynthetic apparatus in Synechococcus elongates and spinach (Spinacia oleracia L.).-Photosynthetica 42: 425-430, 2004. Go to original source...
    28. Parida, A.K., Das, A.B., Mittra, B.: Effects of NaCl stress on the structure, pigment complex composition, and photosynthetic activity of mangrove Bruguiera parviflora chloroplasts.-Photosynthetica 41: 191-200, 2003. Go to original source...
    29. Porta, N.L., Bertamini, M., Nedunchezhian, N., Muthuchelian, K.: High irradiance induced changes of photosystem 2 in young and mature needles of cypress (Cypressus sempervirens L.).-Photosynthetica 42: 263-271, 2004. Go to original source...
    30. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    31. Prasad, S.M., Singh, J.B., Rai, L.C., Kumar, H.D.: Metal-induced inhibition of photosynthetic electron transport chain of the cyanobacterium Nostoc muscorum.-FEMS Microbiol. Lett. 82: 95-100, 1991. Go to original source...
    32. Prasil, O., Adir, N., Ohad, I.: Dynamics of photosystem II: mechanism of photoinhibition and recovery process.-In: Barber, J. (ed.): The Photosystems: Structure, Function and Molecular Biology. Pp. 295-348, Elsevier, Amsterdam-London-New York-Tokyo 1992. Go to original source...
    33. Smith, M.D., Licatalosi, D.D., Thompson, J.E.: Co-association of cytochrome f catabolites and plastid-lipid-associated protein with chloroplast lipid particles.-Plant Physiol. 124: 211-221, 2000. Go to original source...
    34. Stadtman, E.R.: Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions.-Annu. Rev. Biochem. 62: 797-821, 1993. Go to original source...
    35. Suzuki, T., Tada, O., Makimura, M., Tohri, A., Ohta, H., Yamamoto, Y., Enami, I.: Isolation and characterization of oxygen-evolving Photosystem II complexes retaining the PsbO, P and Q proteins from Euglena gracilis.-Plant Cell Physiol. 45: 1168-1175, 2004. Go to original source...
    36. Tiwari, B.S., Bose, A., Ghosh, B.: Photosynthesis in rice under a salt stress.-Photosynthetica 34: 303-306, 1997. Go to original source...
    37. Tripathy, B.C., Bhatia, B., Mohanty, P.: Inactivation of chloroplast photosynthetic electron-transport activity by Ni2+.-Biochim. biophys. Acta 638: 217-224, 1981. Go to original source...
    38. Tripathy, B.C., Mohanty, P.: Zinc-inhibited electron transport of photosynthesis in isolated barley chloroplasts.-Plant Physiol. 66: 1174-1180, 1980. Go to original source...
    39. Wellburn, A.R.: The spectral determination of chlorophylls a and b as well as total carotenoids, using various solvents with spectrophotometers of different resolution.-J. Plant Physiol. 144: 307-313, 1994. Go to original source...

    Return to the content