biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:81-86, 2006 | DOI: 10.1007/s10535-005-0078-6

Polypeptide markers for low temperature stress during seed germination in sunflower

A. Kumar1, S. C. Bhatla1,*
1 Department of Botany, University of Delhi, Delhi, India

Sunflower seeds behaved as chilling and freezing sensitive and also exhibited acclimation under low seed moisture content (< 1 %). At high seed moisture content (approx. 22 %) they tolerated chilling stress but failed to acclimate under freezing temperatures. Pre-imbibitional chilling (5 °C) or freezing (-5 or -10 °C) stress significantly enhanced total soluble protein (TSP) content. Chilling treatment after imbibition (in contrast to pre-imbibition) enhanced germination and this was accompanied by increase in 30, 24 and 21.9 kDa TSPs content (3 d after germination). Freezing at -5 and -10 °C suppressed seed germination and increased content of 78 and 56.2 kDa wall bound proteins. Chilling acclimation decreased 35.4, 33.9, 29.5, 23.4 and 21.4 kDa TSPs.

Keywords: chilling stress; freezing stress; Helianthus annuus L.; protein
Subjects: germination; Helianthus annuus; polypeptide markers; proteins; seed; temperature, low, stress; wall bound protein

Received: February 10, 2004; Accepted: September 3, 2004; Published: March 1, 2006  Show citation

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Kumar, A., & Bhatla, S.C. (2006). Polypeptide markers for low temperature stress during seed germination in sunflower. Biologia plantarum50(1), 81-86. doi: 10.1007/s10535-005-0078-6
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