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Association of SNPs in zinc transporter genes on seminal plasma zinc levels in humans

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Abstract

This study is to examine the effects of single nucleotide polymorphisms (SNPs) of SLC30A and SLC39A on seminal plasma zinc concentration. Blood and seminal plasma samples were collected from outpatients. SNPs of zinc transporters were analyzed by next Generation sequencing technology, and seminal plasma zinc concentration were determined by inductively coupled plasma optical emission spectrometry. Our date showed nine SNPs (SLC30A8 rs2466295, rs2466294, SLC30A10 c.-160 C>G, SLC39A8 rs9331, rs9705, rs151392, rs151393, SLC39A11 rs9912126, SLC39A14 rs1051708) were significantly associated with seminal plasma zinc concentration, and 14 SNPs (SLC30A8 rs2466295, rs2466294, SLC30A10 c.-160 C>G, SLC39A6 rs148550301, SLC39A8 rs9331, rs9705, rs151392, rs151393, SLC39A11 rs9912126, rs61736066, rs36041371 and SLC39A14 rs1051708, rs76963096, rs17060854) were found to be significantly associated with total zinc per ejaculate. The seminal plasma zinc concentrations and total zinc per ejaculate were associated with the number of SNPs, and decreased significantly when five SNPs (SLC39A8 rs9331, rs9705, rs151392, rs151393 and SLC39A14 rs1051708) were a combination of homozygous genotype. Our findings suggest that different zinc transporter SNPs may significantly affect seminal plasma zinc levels.

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Acknowledgements

This study was funded by S&T Program of Heibei (Grant Number [21377796D]), Hebei Natural Science Foundation (Grant Number [H2021314001]), the Human Resources and Social Security Department of Hebei Province (Grant Number [A202101068]) and Hebei Provincial Health Commission (Grant Number [20220796]).

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Author notes

  1. Peipei Deng and Xiaoke Han contributed equally to this work

Authors and Affiliations

  1. NHC Key Laboratory of Family Planning and Healthy, Hebei Key Laboratory of Reproductive Medicine, Hebei Institute of Reproductive Health Science and Technology, No. 480 Heping Street, Xinhua District, Shijiazhuang, 050071, China

    Peipei Deng, Jing Ma & Shusong Wang

  2. Xingtai Reproduction and Genetics Specialist Hospital, Xingtai Infertility Specialist Hospital, 41 Dongguan Street, Xiangdu District, Xingtai, 054000, Hebei Province, China

    Xiaoke Han, Yasong Geng & Bo Zheng

  3. School of Chemistry and Materials Science, Hebei Normal University, Shijiazhuang, China

    Xin Huang

  4. Graduate School of Hebei Medical University, Shijiazhuang, China

    Bo Sun

Authors
  1. Peipei Deng
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  2. Xiaoke Han
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  3. Jing Ma
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  4. Xin Huang
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  5. Bo Sun
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  6. Yasong Geng
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  7. Bo Zheng
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  8. Shusong Wang
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Contributions

All authors contributed to the study conception and design. Material preparation were performed by XH, XH, BS and YG. Data collection and analysis were performed by PD, XH, JM. SW and BZ involved in Protocol development, data analysis and editing the manuscript. The first draft of the manuscript was written by PD. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Bo Zheng or Shusong Wang.

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The authors have no competing interests to declare that are relevant to the content of this article.

Ethical approval

The study protocol was approved by the Ethical Review Board of Hebei Institute of Reproductive health science and technology.

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Informed consent was obtained from all individual participants included in the study.

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Deng, P., Han, X., Ma, J. et al. Association of SNPs in zinc transporter genes on seminal plasma zinc levels in humans. Biometals 35, 955–965 (2022). https://doi.org/10.1007/s10534-022-00416-2

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  • DOI: https://doi.org/10.1007/s10534-022-00416-2

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