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Purification and characterization of mycoferritin from Fusarium verticillioides MRC 826

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Abstract

The fungus Fusarium verticillioides MRC 826 (ascomycetes species), a toxigenic isolate is capable of synthesizing mycoferritin only upon induction with iron in yeast extract sucrose medium. The molecular mass, yield, iron and carbohydrate contents of the purified mycoferritin were 460 kDa, 0.010 mg/g of wet mycelia, 1.0 and 40.2%, respectively. Native gel electrophoresis of the mycoferritin revealed two bands possibly representing isoforms of ferritin. Subunit analysis by SDS–PAGE showed a single protein subunit of ~24 kDa suggesting similar sized subunits in the structure of apoferritin shell. Immunological cross reactivity was observed with the anti-fish liver ferritin. Transmission electron microscopy revealed an apparent particle size of 100 Å. N-terminal amino acid sequencing of mycoferritin showed identities with other eukaryotic ferritin sequences. The spectral characteristics were similar to equine spleen ferritin. However, circular dichroic spectra revealed a higher degree of helicity. Functionally, induction of mycoferritin minimizes the pro-oxidant role of iron.

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Acknowledgments

Ms. Vakdevi was financially supported by a Senior Research Fellowship from the Council of Scientific and Industrial Research, New Delhi, Government of India. The authors also wish to thank Dr. G. Bhanu Prakash Reddy, Assistant Director, National Institute of Nutrition, Hyderabad for CD spectral analysis.

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  1. Department of Biochemistry, University College of Science, Osmania University, Hyderabad, Andhra Pradesh, India

    Vakdevi Validandi, Karuna Rupula, Sashidhar Rao Beedu & Vijay Deshpande

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  1. Vakdevi Validandi
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  2. Karuna Rupula
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  3. Sashidhar Rao Beedu
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  4. Vijay Deshpande
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Correspondence to Vijay Deshpande.

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Validandi, V., Rupula, K., Beedu, S.R. et al. Purification and characterization of mycoferritin from Fusarium verticillioides MRC 826. Biometals 22, 1063–1073 (2009). https://doi.org/10.1007/s10534-009-9257-9

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