Abstract
Objectives
To determine the most efficient design of a hollow fiber-based bioreactor device for a bioartificial liver support system through comparative bioengineering evaluations.
Results
We compared two types of hollow fiber-based bioreactors, the interwoven-type bioreactor (IWBAL) and the dialyzer-type bioreactor (DBAL), by evaluating the overall mass transfer coefficient (K) and the convective coefficient (X). The creatinine and albumin mass transfer coefficients and convective coefficients were calculated using our mathematical model based on the homoporous theory and the modified Powell method. Additionally, using our model, we simulated the mass transport efficiency in clinical-scale BALs. The results of this experiment demonstrate that the mass transfer coefficients for creatinine and albumin increased proportionally with velocity with the IWBAL, and were consistently greater than that found with the DBAL. These differences were further enhanced in the simulation of the large-scale model.
Conclusions
Our findings indicate that the IWBAL with its unique 30° cross hollow fiber design can provide greater solute removal and more efficient metabolism when compared to the conventional DBAL design.
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References
Baquerizo A, Mhoyan A, Kearns-Jonker M, Arnaout WS, Shackleton C, Busuttil RW, Demetriou AA, Cramer DV (1999) Characterization of human xenoreactive antibodies in liver failure patients exposed to pig hepatocytes after bioartificial liver treatment: an ex vivo model of pig to human xenotransplantation. Transplantation 67(1):5–18
Brotherton J, Dar H, Shapour A, Michael M (2007) ELAD cellular and system performance improvements. AASLD, Alexandria
Chari RS, Collins BH, Magee JC, DiMaio JM, Kirk AD, Harland RC, McCann RL, Platt JL, Meyers WC (1994) Brief report: treatment of hepatic failure with ex vivo pig-liver perfusion followed by liver transplantation. N Engl J Med 331(4):234–237
Chopra P, Hao J, Li SK (2010) Iontophoretic transport of charged macromolecules across human sclera. Int J Pharm 388(1–2):107–113
Daugirdas JT (1993) Second generation logarithmic estimates of single-pool variable volume Kt/V: an analysis of error. J Am Soc Nephrol 4(5):1205–1213
Gerlach J, Trost T, Ryan CJ, Meissler M, Hole O, Muller C, Neuhaus P (1994) Hybrid liver support system in a short term application on hepatectomized pigs. Int J Artif Organs 17(10):549–553
Gerlach JC, Mutig K, Sauer IM, Schrade P, Efimova E, Mieder T, Naumann G, Grunwald A, Pless G, Mas A et al (2003) Use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors: a morphologic study. Transplantation 76(5):781–786
Group KDIGOCW (2013) KDIGO 2012 clinical practice guideline for the evaluation and management of chronic kidney disease. Kidney Int Suppl 3:1–150
Hamada H, Sakiyama R, Yamashita AC, Okamoto M, Tojo K (1999) New analytical solution for Pyle–Popovich’s peritoneal dialysis model. J Chem Eng Jpn 32:498–505
Hamada H, Sakiyama R, Yamashita AC, Okamoto M, Tojo KJ, Kumano K, Sakai T (2000) Validation of new analytical solution for Pyle–Popovich’s peritoneal dialysis model. Nephrology 5(1):59–64
Johnston ST, Deen WM (1999) Hindered convection of proteins in agarose gels. J Membr Sci 153(2):271–279
Mamun AA, Hamada H, Karino T, Namoto S, Yamashita AC, Ishizaki M, Okamoto M (2008) Clinical application of computer-aided diagnostic system for harmonious introduction of complementary dialysis therapy. Open Biomed Eng J 2(1):10–16
Matsushita T, Amiot B, Hardin J, Platt JL, Nyberg SL (2003) Membrane pore size impacts performance of a xenogeneic bioartificial liver. Transplantation 76(9):1299–1305
Nedredal GI, Amiot BP, Nyberg P, Luebke-Wheeler J, Lillegard JB, McKenzie TJ, Nyberg SL (2009) Optimization of mass transfer for toxin removal and immunoprotection of hepatocytes in a bioartificial liver. Biotechnol Bioeng 104(5):995–1003
Nyberg SL, Platt JL, Shirabe K, Payne WD, Hu WS, Cerra FB (1992a) Immunoprotection of xenocytes in a hollow fiber bioartificial liver. ASAIO J 38(3):M463–M467
Nyberg SL, Shatford RA, Hu WS, Payne WD, Cerra FB (1992b) Hepatocyte culture systems for artificial liver support: implications for critical care medicine (bioartificial liver support). Crit Care Med 20(8):1157–1168
Nyberg SL, Yagi T, Matsushita T, Hardin J, Grande JP, Gibson LE, Platt JL (2003) Membrane barrier of a porcine hepatocyte bioartificial liver. Liver Transpl 9(3):298–305
Popovich RP, Pyle WK, Bomer JB, Moncrief JW (1979) Peritoneal dialysis—chronic replacement of kidney function. J Chem Eng Jpn 75:31–45
Powell MJD (1964) An efficient method for finding the minimum of a function of several variables without calculating derivatives. Comput J 7(2):155–162
Ratcliffe E, Thomas RJ, Stacey AJ (2014) Visualizing medium and biodistribution in complex cell culture bioreactors using in vivo imaging. Biotechnol Prog 30(1):256–260
Sakiyama R, Ishimori I, Akiba T, Mineshima M (2012) Effect of blood flow rate on internal filtration in a high-flux dialyzer with polysulfone membrane. J Artif Organs 15(3):266–271
Sauer IM, Zeilinger K, Pless G, Kardassis D, Theruvath T, Pascher A, Goetz M, Neuhaus P, Gerlach JC (2003) Extracorporeal liver support based on primary human liver cells and albumin dialysis–treatment of a patient with primary graft non-function. J Hepatol 39(4):649–653
Sterling K (1951) The turnover rate of serum albumin in man as measured by I131-tagged albumin. J Clin Invest 30(11):1228–1237
Acknowledgements
This study was supported by California Institute for Regenerative Medicine Tools and Technologies Awards III RT3-07670 (TM). The authors have received no payment for the preparation of this manuscript and state no other financial and non-financial conflicts of interest.
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Sakiyama, R., Hamada, H., Blau, B. et al. Evaluation of the mass transfer rate using computer simulation in a three-dimensional interwoven hollow fiber-type bioartificial liver. Biotechnol Lett 40, 1567–1578 (2018). https://doi.org/10.1007/s10529-018-2609-1
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DOI: https://doi.org/10.1007/s10529-018-2609-1