Abstract
Objectives
To evaluate the remediation efficiency of Mucor hiemalis by comparing media elimination, uptake, and biotransformation of microcystin-LR with exposure to pure toxin versus a crude bloom extract.
Results
With exposure to the extract, the elimination rate of microcystin-LR from the media, which was 0.28 ng MC-LR l−1 h−1, was significantly higher compared to that achieved with exposure to the pure toxin (0.16 ng MC-LR l−1 h−1) after 24 h. However, intracellular breakdown of microcystin-LR was significantly lower in the extract exposed pellets compared to the pure toxin treated fungal pellets over time. This coincided with reduced intracellular glutathione S-transferase activity with crude extract exposure which could be responsible for the detection of only the glutathione conjugate of microcystin-LR.
Conclusion
This paper signifies the importance of using laboratory exposure scenarios which resemble conditions in nature to fully understand and evaluate remediation efficiency. There is merit in using M. hiemalis for mycoremediation of cyanotoxins in surface waters.
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Acknowledgements
We would like to thank Evelyn Balsano (TU Berlin) and Martina Bessi (University of Florence) for assistance in the research.
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Esterhuizen-Londt, M., Hertel, S. & Pflugmacher, S. Uptake and biotransformation of pure commercial microcystin-LR versus microcystin-LR from a natural cyanobacterial bloom extract in the aquatic fungus Mucor hiemalis . Biotechnol Lett 39, 1537–1545 (2017). https://doi.org/10.1007/s10529-017-2378-2
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DOI: https://doi.org/10.1007/s10529-017-2378-2