Skip to main content
SpringerLink
Log in

Decreased formation of branched-chain short fatty acids in Bacillus amyloliquefaciens by metabolic engineering

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objectives

To reduce the unpleasant odor during 1-deoxynojirimycin (DNJ) production, the genes of leucine dehydrogenase (bcd) and phosphate butryltransferase (ptb) were deleted from Bacillus amyloliquefaciens HZ-12, and the concentrations of branched-chain short fatty acids (BCFAs) and DNJ were compared.

Results

By knockout of the ptb gene, 1.01 g BCFAs kg−1 was produced from fermented soybean by HZ-12Δptb. This was a 56% decrease compared with that of HZ-12 (2.27 g BCFAs kg−1). Moreover, no significant difference was found in the DNJ concentration (0.7 g kg−1). After further deletion of the bcd gene from HZ-12Δptb, no BCFAs was detected in fermented soybeans with HZ-12ΔptbΔbcd, while the DNJ yield decreased by 26% compared with HZ-12.

Conclusions

HZ-12Δptb had decreased BCFAs formation but also maintained the stable DNJ yield, which contributed to producing DNJ-rich products with decreased unpleasant smell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Cai D, Liu M, Wei X, Li X, Wang Q, Nomura CT, Chen S (2016) Use of Bacillus amyloliquefaciens HZ-12 for high-level production of the blood glucose lowering compound, 1-deoxynojirimycin (DNJ), and nutraceutical enriched soybeans via fermentation. Appl Biochem. doi:10.1007/s12010-016-2272-8

    Google Scholar 

  • Cho Y, Park Y, Lee J, Kang K, Hwang K, Seong S (2008) Hypoglycemic effect of culture broth of Bacillus subtilis S10 producing 1-deoxynojirimycin. J Korean Soc Food Sci Nutr 37:1401–1407

    Article  CAS  Google Scholar 

  • Kang K et al (2011) Identification of the genes involved in 1-deoxynojirimycin synthesis in Bacillus subtilis MORI 3 K-85. J Microbiol 49:431–440

    Article  CAS  PubMed  Google Scholar 

  • Liu Q, Li X, Li C, Zheng Y, Wang F, Li H, Peng G (2016) 1-Deoxynojirimycin alleviates liver injury and improves hepatic glucose metabolism in db/db mice. Molecules 21:279

    Article  PubMed  Google Scholar 

  • Onose S, Ikeda R, Nakagawa K, Kimura T, Yamagishi K, Higuchi O, Miyazawa T (2013) Production of the α-glycosidase inhibitor 1-deoxynojirimycin from Bacillus species. Food Chem 138:516–523

    Article  CAS  PubMed  Google Scholar 

  • Paek N, Kang D, Choi Y, Lee J, Kim T, Kim K (1997) Production of 1-deoxynojirimycin by Streptomyces sp. SID9135. J Microbiol Biotechnol 7:262–266

    CAS  Google Scholar 

  • Qi G et al (2014) Deletion of meso-2,3-butanediol dehydrogenase gene budC for enhanced D-2,3-butanediol production in Bacillus licheniformis. Biotechnol Biofuel 7:16

    Article  Google Scholar 

  • Qiu Y, Xiao F, Wei X, Wen Z, Chen S (2014) Improvement of lichenysin production in Bacillus licheniformis by replacement of native promoter of lichenysin biosynthesis operon and medium optimization. Appl Microbiol Biotechnol 98:8895–8903

    Article  CAS  PubMed  Google Scholar 

  • Seo MJ, Nam YD, Lee SY, Park SL, Yi SH, Lim SI (2013) Isolation of the putative biosynthetic gene cluster of 1-deoxynojirimycin by Bacillus amyloliquefaciens 140 N, its production and application to the fermentation of soybean paste. Biosci Biotechnol Biochem 77:398–401

    Article  CAS  PubMed  Google Scholar 

  • Sirobhushanam S, Galva C, Sen S, Wilkinson BJ, Gatto C (2016) Broad substrate specificity of phosphotransbutyrylase from Listeria monocytogenes: a potential participant in an alternative pathway for provision of acyl CoA precursors for fatty acid biosynthesis. Biochim Biophys Acta 1861:1102–1110

    Article  CAS  PubMed  Google Scholar 

  • Smit BA, Engels WJ, Smit G (2009) Branched chain aldehydes: production and breakdown pathways and relevance for flavour in foods. Appl Microbiol Biotechnol 81:987–999

    Article  CAS  PubMed  Google Scholar 

  • Takemura H, Ando N, Tsukamoto Y (2000) Breeding of branched short-chain fatty acids non-producing natto bacteria and its application to production of natto with light smells. J Jpn Soc Food Sci 47:773–779

    Article  CAS  Google Scholar 

  • Yatsunami K, Ichida M, Onodera S (2008) The relationship between 1-deoxynojirimycin content and alpha-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.) in Kyoto Japan. J Nat Med 62:63–66

    Article  CAS  PubMed  Google Scholar 

  • Yu M, Zhang Y, Tang IC, Yang ST (2011) Metabolic engineering of Clostridium tyrobutyricum for n-butanol production. Metab Eng 13:373–382

    Article  CAS  PubMed  Google Scholar 

  • Zhou L, Fang L, Sun Y, Su Y, Zhu W (2016) Effects of the dietary protein level on the microbial composition and metabolomic profile in the hindgut of the pig. Anaerobe 38:61–69

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by the National Natural Science Foundation of China (31501468) and the Fundamental Research Funds for the Central Universities (2662016PY121).

Author information

Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    Yangyang Chen, Mengjie Liu, Shouwen Chen & Xuetuan Wei

  2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Xuetuan Wei

  3. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Yangyang Chen

Authors
  1. Yangyang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mengjie Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shouwen Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xuetuan Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xuetuan Wei.

Ethics declarations

Supporting information

Supplementary Table 1—Strains and plasmids used.

Supplementary Table 2—Primers used for PCR.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 27 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Y., Liu, M., Chen, S. et al. Decreased formation of branched-chain short fatty acids in Bacillus amyloliquefaciens by metabolic engineering. Biotechnol Lett 39, 529–533 (2017). https://doi.org/10.1007/s10529-016-2270-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-016-2270-5

Keywords

Search

Navigation