Abstract
Objective
To produce δ-decalactone from linoleic acid by one-pot reaction using linoleate 13-hydratase with supplementation with whole Yarrowia lipolytica cells.
Results
Whole Y. lipolytica cells at 25 g l−1 produced1.9 g l−1 δ-decalactone from 7.5 g 13-hydroxy-9(Z)-octadecenoic acid l−1 at pH 7.5 and 30 °C for 21 h. Linoleate 13-hydratase from Lactobacillus acidophilus at 3.5 g l−1 with supplementation with 25 g Y. lipolytica cells l−1 in one pot at 3 h produced 1.9 g l−1 δ-decalactone from 10 g linoleic acid l−1 via 13-hydroxy-9(Z)-octadecenoic acid intermediate at pH 7.5 and 30°C after 18 h, with a molar conversion yield of 31 % and productivity of 106 mg l−1 h−1.
Conclusion
To the best of our knowledge, this is the first production of δ-decalactone using unsaturated fatty acid.
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Acknowledgments
This work was supported by a Grant from the Korea Healthcare Technology R and D Project, Ministry for Health and Welfare, Republic of Korea (No. 2012-009).
Supporting information
Supplementary Fig. 1—GC/MS spectrum of the reaction product δ-decalactone obtained from 13-hydroxy-9(Z)-octadecenoic acid by whole Y. lipolytica cells.
Supplementary Fig. 2—Effects of temperature and pH on the conversion of 13-hydroxy-9(Z)-octadecenoic acid to δ-decalactone by whole Y. lipolytica cells.
Supplementary Fig. 3—Effect of the concentration of linoleic acid on the production of δ-decalactone from 13-hydroxy-9(Z)-octadecenoic acid by whole Y. lipolytica cells.
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Kang, WR., Seo, MJ., An, JU. et al. Production of δ-decalactone from linoleic acid via 13-hydroxy-9(Z)-octadecenoic acid intermediate by one-pot reaction using linoleate 13-hydratase and whole Yarrowia lipolytica cells. Biotechnol Lett 38, 817–823 (2016). https://doi.org/10.1007/s10529-016-2041-3
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DOI: https://doi.org/10.1007/s10529-016-2041-3