Skip to main content
Log in

The use of chitooligosaccharide in beer brewing for protection against beer-spoilage bacteria and its influence on beer performance

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objectives

To identify a biological preservative that can protect beer from microbial contamination, which often results in the production of turbidity and off-flavor.

Results

The antimicrobial activity of a chitooligosaccharide against beer-spoilage bacteria and its effect on the fermentation performance of brewer’s yeast was studied. Chitooligosaccharide with an average 2 kDa molecular weight was the best at inhibiting all tested beer-spoilage bacteria. The application of chitooligosaccharide in the brewing process did not influence the fermentation of brewer’s yeast. The change in beer performance induced by the contamination of Lactobacillus brevis could be effectively controlled by application of chitooligosaccharide in the beer brewing process.

Conclusion

The experimental data suggested that chitooligosaccharide should be an excellent preservative to inhibit beer-spoilage bacteria in the brewing process and in the end product.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • European Brewery Convention (EBC) (2004) Analytica-EBC. Verlag Hans Getränke-Fachverlag, Nürnberg

    Google Scholar 

  • Garg P, Park YJ, Sharma D, Wang T (2010) Antimicrobial effect of chitosan on the growth of lactic acid bacteria strains known to spoil beer. J Exper Microb Immunol 14:7–12

    Google Scholar 

  • Gil G, del Mónaco S, Cerrutti P, Galvagno M (2004) Selective antimicrobial activity of chitosan on beer spoilage bacteria and brewing yeasts. Biotechnol Lett 26:569–572

    Article  CAS  PubMed  Google Scholar 

  • Gómez-Rivas L, Escudero-Abarca BI, Aguilar-Uscanga MG, Hayward-Jones PM, Mendoza P, Ramires M (2004) Selective antimicrobial action of chitosan against spoilage yeasts in mixed culture fermentations. J Ind Microbiol Biotechnol 31:16–22

    Article  PubMed  Google Scholar 

  • Hollerová I, Kubizniaková P (2001) Monitoring Gram positive bacterial contamination in Czech breweries. J Inst Brew 107:355–358

    Article  Google Scholar 

  • Jeon YJ, Park PJ, Kim SK (2001) Antimicrobial effect of chitooligosaccharides produced by bioreactor. Carbohyd Polym 44:71–76

    Article  CAS  Google Scholar 

  • Lei H, Zhao H, Yu Z, Zhao M (2012) Effect of wort gravity and nitrogen level on fermentation performance of brewer’s yeast and the formation of flavour volatiles. Appl Biochem Biotechnol 166:1562–1574

    Article  CAS  PubMed  Google Scholar 

  • Li X, Chen X (2007) Biodegradation of polysaccharide sourced from virulence factor or plant and pathogenic cell wall constituent and its application in management of phytopathogenic disease. In: Environmental Biodegradation Research Focus, Edited by BY Wang. Nova Science Publishers, Inc. pp. 1–47

  • No HK, Park NY, Lee SH, Meyers SP (2002) Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int J Food Microbiol 74:65–72

    Article  CAS  PubMed  Google Scholar 

  • Pfeiffer JR, Oliver JM, Wilson BS (2002) Observing signal transduction, endocytosis and degranulation by immunogold labeling and transmission electron microscopy on membrane sheets. Am Biotech Meth 20:18–22

    CAS  Google Scholar 

  • Pulusani SR, Rao DR, Sunki GR (1979) Antimicrobial activity of lactic cultures: partial purification and characterization of antimicrobial compound(s) produced by Streptococcus thermophilus. J Food Sci 44:575–578

    Article  CAS  Google Scholar 

  • Silvetti T, Brasca M, Lodi R, Vanoni L, Chiolerio F, Groot M, Bravi A (2010) Effects of lysozyme on the microbiological stability and organoleptic properties of unpasteurized beer. J Inst Brew 116:33–40

    Article  CAS  Google Scholar 

  • Suzuki K (2011) Microbiological instability of beer caused by spoilage bacteria. J Inst Brew 117:131–155

    Article  CAS  Google Scholar 

  • Suzuki K, Iijima K, Sakamoto K, Sami M, Yamashita H (2006) A review of hop resistance in beer spoilage lactic acid bacteria. J Inst Brew 112:173–191

    Article  CAS  Google Scholar 

  • Tang T, Shi T, Qian K, Li P, Li J, Cao Y (2009) Determination of biogenic amines in beer with pre-column derivatization by high performance liquid chromatography. J Chromatogr B 877:507–512

    Article  CAS  Google Scholar 

  • Vriesekoop F, Krahl M, Hucker B, Menz G (2012) Bacteria in brewing: the good, the bad and the ugly. J Inst Brew 118:335–345

    Article  Google Scholar 

  • Yu Z, Zhao H, Li H, Zhang Q, Lei H, Zhao M (2012a) Selection of Saccharomyces pastorianus variants with improved fermentation performance under very high gravity wort conditions. Biotechnol Lett 34:365–370

    Article  CAS  PubMed  Google Scholar 

  • Yu Z, Zhao H, Zhao M, Lei H, Li H (2012b) Metabolic flux and nodes control analysis of brewer’s yeasts under different fermentation temperature during beer brewing. Appl Biochem Biotechnol 168:1938–1952

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Natural Science Foundation of China (31401681) and the Science and Technology Foundation of Dalian (2013J21DW007).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Xianzhen Li.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, X., Yu, Z., Wang, T. et al. The use of chitooligosaccharide in beer brewing for protection against beer-spoilage bacteria and its influence on beer performance. Biotechnol Lett 38, 629–635 (2016). https://doi.org/10.1007/s10529-015-2013-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-015-2013-z

Keywords

Navigation