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Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes

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Abstract

Objectives

To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures.

Results

An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S. cerevisiae strains, a laboratory strain (CEN.PK2-1C) and an industrial strain (Kyokai No. 7). The engineered Kyokai No. 7 strain (K7-XYL) exhibited a higher sugar consumption rate (1.03 g l−1 h−1) and ethanol yield (63.8 %) from a glucose and xylose mixture compared to the engineered CEN.PK2-1C strain. Furthermore, K7-XYL produced a larger amount of ethanol (39.6 g l−1) compared to K7-SsXYL (32 g l−1) with integrated xylose reductase and xylitol dehydrogenase from a xylose-assimilating yeast Scheffersomyces stipitis instead of GRE3 and SOR1.

Conclusion

The created S. cerevisiae strain showed sufficient xylose-fermenting ability to be used for efficient ethanol production from glucose/xylose.

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Authors and Affiliations

  1. Bio Fuels & Chemicals R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JX Nippon Oil & Energy Corporation, 8, Chidori-cho, Naka-Ku, Yokohama, 231-0815, Japan

    Jin Konishi, Akira Fukuda, Kozue Mutaguchi & Takeshi Uemura

Authors
  1. Jin Konishi
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  2. Akira Fukuda
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  3. Kozue Mutaguchi
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  4. Takeshi Uemura
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Correspondence to Jin Konishi.

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Konishi, J., Fukuda, A., Mutaguchi, K. et al. Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes. Biotechnol Lett 37, 1623–1630 (2015). https://doi.org/10.1007/s10529-015-1840-2

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  • DOI: https://doi.org/10.1007/s10529-015-1840-2

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