Skip to main content
SpringerLink
Log in

Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fusion of the PHA synthase, PhaC, to lipase B yielded active PHA lipase beads capable of hydrolyzing glycerol tributyrate. Lipase B beads showed stable activity over several weeks and re-usability without loss of function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Chen B, Hu J, Miller EM, Xie W, Cai M, Gross RA (2008) Candida antarctica lipase B chemically immobilized on epoxy-activated micro—and nanobeads: catalysts for polyester synthesis. Biomacromolecules 9:463–471

    Article  CAS  PubMed  Google Scholar 

  • Goodman LP, Dugan LR Jr (1969) The effect of sonication on lipase activity. Lipids 5:362–365

    Article  Google Scholar 

  • Grage K, Jahns AC, Parlane N, Palanisamy R, Rasiah IA, Atwood JA, Rehm BHA (2009) Bacterial polyhydroxyalkanoate granules: biogenesis, structure, and potential use as nano-/micro-beads in biotechnological and biomedical applications. Biomacromolecules 10:660–669

    Article  CAS  PubMed  Google Scholar 

  • Jaeger K-E, Reetz MT (1998) Microbial lipases form versatile tools for biotechnology. Trends Biotechnol 16:396–403

    Article  CAS  PubMed  Google Scholar 

  • Jahns AC, Haverkamp RG, Rehm BHA (2008) Multifunctional inorganic-binding beads self-assembled inside engineered bacteria. Bioconjug Chem 19:2072–2080

    Article  CAS  PubMed  Google Scholar 

  • Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Ferndandez-Lafuente R (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol 40:1451–1463

    Article  CAS  Google Scholar 

  • Mei Y, Miller L, Gao W, Gross RA (2003) Imaging the distribution and secondary structure of immobilized enzymes using infrared microspectroscopy. Biomacromolecules 4:70–74

    Article  CAS  PubMed  Google Scholar 

  • Rathi P, Saxena RK, Gupta R (2001) A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Process Biochem 37:187–192

    Article  CAS  Google Scholar 

  • Rehm BHA (2003) Polyester synthases: natural catalysts for plastics. Biochem J 376:15–33

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Rehm BHA (2006) Genetics and biochemistry of polyhydroxyalkanoate granule self-assembly: the key role of polyester synthases. Biotechnol Lett 28:207–213

    Article  CAS  PubMed  Google Scholar 

  • Sheldon RA (2007) Enzyme immobilization: the quest for optimum performance. Adv Synth Catal 349:1289–1307

    Article  CAS  Google Scholar 

  • Svendsen A, Clausen IG, Patkar SA, Borch K, Thellersen M (1997) Protein engineering of microbial lipases of industrial interest. Methods Enzymol 284:317–340

    Article  CAS  Google Scholar 

  • Tan T, Hu J, Nie K, Deng L, Wang F (2010) Biodiesel production with immobilized lipase: a review. Biotechnol Adv 28:628–634

    Article  CAS  PubMed  Google Scholar 

  • Uppenberg J, Hansen MT, Patkar S, Jones TA (1994) The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica. Sequence 2:293–308

    CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by PolyBatics Ltd.

Conflict of interest

Bernd Rehm is Chief Science Officer as well as shareholder of Polybatics Ltd.

Supporting information

Supplementary Table 1—Bacterial strains, plasmids and oligonucleotides used in this study.

Author information

Author notes

  1. Anika C. Jahns

    Present address: Department of Medical Biosciences, Umeå University, 90185, Umeå, Sweden

Authors and Affiliations

  1. Institute of Fundamental Sciences, Massey University, Private Bag 11222, Palmerston North, 4442, New Zealand

    Anika C. Jahns & Bernd H. A. Rehm

  2. PolyBatics Ltd, BioCommerce Centre, Dairy Farm Road, Palmerston North, 4442, New Zealand

    Anika C. Jahns & Bernd H. A. Rehm

  3. MacDiarmid Institute for Advanced Materials and Nanotechnology, Kelburn Parade, Wellington, 6140, New Zealand

    Bernd H. A. Rehm

Authors
  1. Anika C. Jahns
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bernd H. A. Rehm
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bernd H. A. Rehm.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 16 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jahns, A.C., Rehm, B.H.A. Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions. Biotechnol Lett 37, 831–835 (2015). https://doi.org/10.1007/s10529-014-1735-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-014-1735-7

Keywords

Search

Navigation