Abstract
Protoplast fusion was used to obtain a higher production of lignocellulolytic enzymes with protoplast fusion in Trichoderma reesei. The fusant strain T. reesei JL6 was obtained from protoplast fusion from T. reesei strains QM9414, MCG77, and Rut C-30. Filter paper activity of T. reesei JL6 increased by 18 % compared with that of Rut C-30. β-Glucosidase, hemicellulase and pectinase activities of T. reesei JL6 were also higher. The former activity was 0.39 Uml−1, while those of QM9414, MCG77, and Rut C-30 were 0.13, 0.11, and 0.16 Uml−1, respectively. Pectinase and hemicellulase activities of JL6 were 5.4 and 15.6 Uml−1, respectively, which were slightly higher than those of the parents. The effects of corn stover and wheat bran carbon sources on the cellulase production and growth curve of T. reesei JL6 were also investigated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adsul MG, Bastawde KB, Varma AJ, Gokhale DV (2007) Strain improvement of Penicillium janthinellum NCIM 1171 for increased cellulase production. Bioresour Technol 98:1467–1473
Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23:257–270
Esterbauer H, Steiner W, Labudova I, Hermann A, Hayn M (1991) Production of Trichoderma cellulase in laboratory and pilot scale. Bioresour Technol 36:51–65
Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268
Jørgensen H, Kristensen JB, Felby C (2007) Enzymatic conversion of lignocellulose into fermentable sugars: challenges and opportunities. Biofuel Bioprod Biorefining 1:119–134
Klug-Santner BG, Schnitzhofer W, Vršanská M, Weber J, Agrawal PB, Nierstrasz VA, Guebitz GM (2006) Purification and characterization of a new bioscouring pectatelyase from Bacillus pumilus BK2. J Biotechnol 121:390–401
Le DG, Mounier J, Vasseur V, Arzur D, Habrylo O, Barbier G (2010) Quantification of Penicillium camemberti and P.roqueforti mycelium by real-time PCR to assess their growth dynamics during ripening cheese. Int J Food Microbiol 138:100–107
Martinez D, Berka RM, Henrissat B et al (2008) Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nat Biotechnol 26:553–560
Prabavathy VR, Mathivanan N, Sagadevan E, Murugesan K, Lalithakumari D (2006) Self-fusion of protoplasts enhances chitinase production and biocontrol activity in Trichoderma harzianum. Bioresour Technol 97:2330–2334
Sheir-Neiss G, Montenecourt BS (1984) Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations. Appl Microbiol Biotechnol 20:46–53
Stephanopoulos G (2002) Metabolic engineering by genome shuffling. Nat Biotechnol 20:666–668
Vinzant TB, Adney WS, Decker SR, Baker JO, Kinter MT, Sherman NE, Fox JW, Himmel ME (2001) Fingerprinting Trichoderma reesei hydrolases in a commercial cellulase preparation. Appl Biochem Biotechnol 91:99–107
Zhang PYH, Himmel ME, Mielenz JR (2006) Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24:452–481
Acknowledgments
This Project was supported by the Jilin Province Development and Reform Commission (2011003-1) and the Program for the 12th Five-Year Plan (grant 2012ZX10001003).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Cui, Yx., Liu, Jj., Liu, Y. et al. Protoplast fusion enhances lignocellulolytic enzyme activities in Trichoderma reesei . Biotechnol Lett 36, 2495–2499 (2014). https://doi.org/10.1007/s10529-014-1627-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-014-1627-x