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Permeabilized probiotic Lactobacillus plantarum as a source of β-galactosidase for the synthesis of prebiotic galactooligosaccharides

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Abstract

Permeabilized probiotic Lactobacillus plantarum was used as a source of β-galactosidase for the synthesis of galactooligosaccharides (GOS) from lactose. β-galactosidase activity was highest when galactose (1,724 Miller Units) was used as a carbon source compared to lactose, sucrose or glucose at 37 °C, 18 h. Permeabilized cells had the highest transgalactosylation activity resulting in 34 % (w/w) GOS synthesis from 40 % (w/v) lactose at 50 °C over 12 h. HPLC revealed that the GOS were composed of 13 % disaccharides (non-lactose), 17 % trisaccharides and 4 % tetrasaccharides that were further confirmed by ESI–MS.

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References

  • Aachary AA, Prapulla SG (2008) Corn-induced endo-1, 4-β-D-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan. J Agric Food Chem 56:3981–3988

    Article  CAS  PubMed  Google Scholar 

  • Cho YJ, Shin HJ, Bucke C (2003) Purification and biochemical properties of a galactooligosaccharides producing β-galactosidase from Bullera singularis. Biotechnol Lett 25:2107–2111

    Article  CAS  PubMed  Google Scholar 

  • Fuller R (1989) Probiotics in man and animals. J Appl Bacteriol 66:365–378

    Article  CAS  PubMed  Google Scholar 

  • Fuller R (1991) Probiotics in human medicine. Gut 32:439–442

    Article  CAS  PubMed  Google Scholar 

  • Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412

    CAS  PubMed  Google Scholar 

  • Hsu CA, Yu RC, Chou CC (2006) Purification and characterization of a sodium-stimulated β-galactosidase from Bifidobacterium longum CCRC15708. World J Microbiol Biotechnol 22:355–361

    Article  Google Scholar 

  • Hung MN, Lee BH (2002) Purification and characterization of a recombinant β-galactosidase with transgalactosylation activity from Bifidobacterium infantis HL96. Appl Microbiol Biotechnol 58:439–445

    Article  CAS  PubMed  Google Scholar 

  • Iwasaki KI, Nakajima M, Nakao SI (1996) Galacto-oligosaccharide production from lactose by an enzymic batch reaction using β-galactosidase. Process Biochem 31:69–76

    Article  CAS  Google Scholar 

  • Kim JW, Rajagopal SN (2000) Isolation and characterization of β-galactosidase from Lactobacillus crispatus. Folia Microbiol 45:29–34

    Article  CAS  Google Scholar 

  • Laxmi NP, Mutamed MA, Nagendra PS (2011) Effect of carbon and nitrogen sources on growth of Bifidobacterium animalis Bb12 and Lactobacillus delbrueckiis sp. Bulgaricus ATCC 11842 and production of β-galactosidase under different culture conditions. Int Food Res J 18:373–380

    CAS  Google Scholar 

  • Madhu AN, Giribhatanavar P, Narayan MS, Prapulla SG (2010) Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC–MS, immunological and microbiological techniques. Biotechnol Lett 32:503–506

    Article  CAS  PubMed  Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Reddy PKKB, Raghavendra P, Girishkumar B, Misra MC, Prapulla SG (2007) Screening of probiotic properties of lactic acid bacteria isolated from Kanjika, an ayurvedic lactic acid fermented product: an in vivo evaluation. J Gen Appl Microbiol 53:207–213

    Article  CAS  PubMed  Google Scholar 

  • Shin HJ, Yang JW (1998) Enzymatic production of galactooligosaccharides by Bullera singularis β-galactosidase. J Microbiol Biotechnol 8:484–489

    CAS  Google Scholar 

  • Somkuti GA, Steinberg DH (1994) Permeabilization of Streptococcus thermophilus and the expression of beta-galactosidase. Enzym Microb Technol 16:573–576

    Article  CAS  Google Scholar 

  • Torres DPM, Goncalves MPF, Teixeira JA, Rodrigues LR (2010) Galacto-oligosaccharides: production, properties, applications, and significance as prebiotics. Compr Rev Food Sci Food Saf 9:438–454

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Fermentation Technology and Bioengineering Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

    Duraiswamy Gobinath & Siddalingaiya Gurudutt Prapulla

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  1. Duraiswamy Gobinath
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  2. Siddalingaiya Gurudutt Prapulla
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Correspondence to Siddalingaiya Gurudutt Prapulla.

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Gobinath, D., Prapulla, S.G. Permeabilized probiotic Lactobacillus plantarum as a source of β-galactosidase for the synthesis of prebiotic galactooligosaccharides. Biotechnol Lett 36, 153–157 (2014). https://doi.org/10.1007/s10529-013-1345-9

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  • DOI: https://doi.org/10.1007/s10529-013-1345-9

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