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Immobilization of β-glucosidase on bifunctional periodic mesoporous organosilicas

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Abstract

The aminopropyl-functionalized ethane-bridged bifunctional periodic mesoporous organosilicas (APEPMOs) were synthesized by the co-condensation of 1,2-bis (triethoxysilyl) ethane and 3-aminopropyltriethoxysilane in the presence of cationic surfactants octadecyltrimethylammonium chloride in basic medium. The pores of the APEPMOs were expanded with N,N-dimethyldecylamine and the pore-expanded materials were utilized as supports for β-glucosidase immobilization. A high enzyme loading of 120 mg per g support was achieved in 18 h, and 95.5 % of enzymatic activity was retained. β-Glucosidases were strongly immobilized on APEPMOs with only 5 % desorption in the washing step with buffer solution. The immobilized enzyme had 75 % activity after 20 batch reactions and had improved thermal stability, relative to the free enzyme. These results demonstrate that APEPMOs would be promising supports for β-glucosidases immobilization.

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Abbreviations

APEPMOs:

Aminopropyl-functionalized ethane-bridged, bifunctional periodic mesoporous organosilicas

PMOs:

Periodic mesoporous organosilanes

BPMOs:

Bifunctional periodic mesoporous organosilanes

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (81173022).

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China

    Liye Guan, Bin Di, Mengxiang Su & Jie Qian

  2. Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, 210009, China

    Bin Di

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  1. Liye Guan
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  2. Bin Di
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  4. Jie Qian
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Correspondence to Bin Di.

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Guan, L., Di, B., Su, M. et al. Immobilization of β-glucosidase on bifunctional periodic mesoporous organosilicas. Biotechnol Lett 35, 1323–1330 (2013). https://doi.org/10.1007/s10529-013-1208-4

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  • DOI: https://doi.org/10.1007/s10529-013-1208-4

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