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In silico analysis of amino acid biosynthesis and proteolysis in Lactobacillus delbrueckii subsp. bulgaricus 2038 and the implications for bovine milk fermentation

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Abstract

The amino acid biosynthesis pathway and proteolytic system of Lactobacillus delbrueckii subsp. bulgaricus 2038 (L. bulgaricus 2038), a mainstay of large-scale yogurt production, were modeled based on its genomic sequence. L. bulgaricus 2038 retains more potential for amino acid synthesis and a more powerful proteolytic system than other L. bulgaricus strains, but favors amino acid uptake over de novo synthesis. Free amino acids and peptides in bovine milk provide the main nitrogen sources; whey is more important than casein for L. bulgaricus during fermentation. Free amino acids are imported by amino acid permeases and by ABC-type transport systems whereas exogenous oligopeptides are imported by ABC-type proteins only. Histidine is neither synthesized nor imported singly, which might explain why L. bulgaricus cannot grow in synthetic media.

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Acknowledgments

This work was sponsored by the Shanghai Rising-Star Program (11QA1404600) and the Shanghai National Science Foundation (11ZR1425500). We are grateful to Dr. Susanne Wagner (Myriad Genetics, Inc.) for her kind help in improving the manuscript.

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Authors and Affiliations

  1. Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, 250 Bi-Bo Road, Zhang Jiang Hi-Tech Park, Shanghai, 201203, China

    Huajun Zheng

  2. Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino Fujisawa, Kanagawa, 252-0880, Japan

    Enuo Liu & Munehiro Oda

  3. Key Laboratory of Systems Biology/Synthetic Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Pei Hao

  4. Division of Research and Development, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa, 250-0862, Japan

    Enuo Liu, Tomonobu Konno & Zai-Si Ji

  5. WHO Collaborating Centre for Research in Human Reproduction, 2140 Xie-Tu Road, Shanghai, 200023, China

    Huajun Zheng & Zai-Si Ji

Authors
  1. Huajun Zheng
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  2. Enuo Liu
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  3. Pei Hao
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  4. Tomonobu Konno
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  5. Munehiro Oda
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  6. Zai-Si Ji
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Corresponding author

Correspondence to Zai-Si Ji.

Additional information

Huajun Zheng, Enuo Liu are the authors contributed equally to this work.

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Zheng, H., Liu, E., Hao, P. et al. In silico analysis of amino acid biosynthesis and proteolysis in Lactobacillus delbrueckii subsp. bulgaricus 2038 and the implications for bovine milk fermentation. Biotechnol Lett 34, 1545–1551 (2012). https://doi.org/10.1007/s10529-012-1006-4

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  • DOI: https://doi.org/10.1007/s10529-012-1006-4

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