Abstract
Based on the newly-released genomic data of Mucor circinelloides CBS 277.49, we have annotated five genes encoding for malic enzyme: all code for proteins that contain conserved domains/motifs for malic acid binding, NAD+ binding and NAD(P)+ binding. Phylogenetic analysis for malic enzyme genes showed that genes ID 78524 and 11639 share ~80% amino acid identity and are grouped in cluster 1; genes ID 182779, 186772 and 116127 share ~66% amino acid identity are grouped in cluster 2. Genes ID 78524, 11639 and 166127 produce proteins that are localized in the mitochondrion, while the products from genes 182779 and 186772 are localized in the cytosol. Based on the comparative analysis published previously by Song et al. (Microbiology 147:1507–1515, 2001), we propose that malic enzyme genes ID 78524, 166127, 182779, 186772, 11639, respectively, represent protein isoforms I, II, III/IV, V, and VI.
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Acknowledgments
The Mc. circinelloides sequence data was provided by the US Department of Energy Joint Genome Institute (JGI) at http://www.jgi.doe.gov/ in collaboration with the user community. Yuanda Song is supported by National Science Foundation of China grant 81071685, Open Project Program of State Key Laboratory of Food Science and Technology, Jiangnan University (No. 2060204), and starting grant from Jiangnan University. Wanwipa Vongsangnak is supported by initial funding from Soochow University. CR is grateful to Jiangnan University for support as a visiting professor.
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Vongsangnak, W., Zhang, Y., Chen, W. et al. Annotation and analysis of malic enzyme genes encoding for multiple isoforms in the fungus Mucor circinelloides CBS 277.49. Biotechnol Lett 34, 941–947 (2012). https://doi.org/10.1007/s10529-012-0859-x
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DOI: https://doi.org/10.1007/s10529-012-0859-x