Abstract
The main toxicity mechanism of Lysinibacillus sphaericus, which is used in the control of mosquitoes, is its binary toxin produced during sporulation; additionally the Mtx1, Mtx2 and Mtx 3 toxins are expressed in vegetative cells. Mosquito larvicidal potency of the S-layer protein that is expressed in vegetative cells has been determined. The protein is similar to other S-layer proteins of mosquitocidal L. sphaericus strains. The LC50 values of the S-layer protein of the L. sphaericus OT4b25, OT4b26, and III(3)7 strains against third-instar larvae of Culex quinquefasciatus were 8.7, 24 and 0.68 μg/ml, respectively. To our knowledge this is the first study showing the mosquito larvicidal potency of the S-layer protein from Lysinibacillus sphaericus.
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Acknowledgments
This work was supported by grants from Colciencias contract RC-295-2008, the Research Committee of the Science Faculty, at the Universidad de los Andes, Colombia. Institutional help from “Fundación Ramon Areces” to the Centro de Biología Molecular “Severo Ochoa” is acknowledged, as is funding to J.A.A. from the Spanish Ministry of Science, and Innovation Grant BFU2009-09200.
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Lozano, L.C., Ayala, J.A. & Dussán, J. Lysinibacillus sphaericus S-layer protein toxicity against Culex quinquefasciatus. Biotechnol Lett 33, 2037–2041 (2011). https://doi.org/10.1007/s10529-011-0666-9
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DOI: https://doi.org/10.1007/s10529-011-0666-9