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Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor

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Abstract

Embryoid body (EB) formation is a common intermediate during in vitro differentiation of pluripotent stem cells into specialized cell types. We have optimized the slow-turning, lateral vessel (STLV) for large scale and homogenous EB production from mouse embryonic stem cells. The effects of inoculating different cell numbers, time of EB adherence to gelatin-coated dishes, and rotation speed for optimal EB formation and cardiac differentiation were investigated. Using 3 × 105 cells/ml, 10 rpm rotary speed and plating of EBs onto gelatin-coated surfaces three days after culture, were the best parameters for optimal size and EB quality on consequent cardiac differentiation. These optimized parameters enrich cardiac differentiation in ES cells when using the STLV method.

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Acknowledgments

This study was supported by EU Seventh Framework Programme (PartnErS, PIAP-GA-2008-218205; InduHeart, PEOPLE-IRG-2008-234390; InduStem, PIAP-GA-2008-230675; PluriSys, HEALTH-2007-B-223485; Resolve, FP7-HEALTH-F4-2008-202047); NKTH-OTKA-EU FP7-HUMAN-2009-MB08-C 80205 and NKTH/KPI (NKFP_07_1-ES2HEART-HU OM-00202-2007), CHE-TRF senior scholarship No. RTA 5080010 (M.T.) and the Thailand Commission on Higher Education (CHE-PhD-SW-2005-100 (S.R.), CHE-PhD-SW-2007 (N.K.)).

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Authors and Affiliations

  1. BioTalentum Ltd., Aulich Lajos u. 26, 2100, Godollo, Hungary

    Sasitorn Rungarunlert, Nuttha Klincumhom, Istvan Bock, Csilla Nemes, Melinda K. Pirity & Andras Dinnyes

  2. Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Sasitorn Rungarunlert, Nuttha Klincumhom & Mongkol Techakumphu

  3. Molecular Animal Biotechnology Laboratory, Szent Istvan University, 2100, Gödöllö, Hungary

    Istvan Bock & Andras Dinnyes

Authors
  1. Sasitorn Rungarunlert
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  2. Nuttha Klincumhom
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  3. Istvan Bock
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  4. Csilla Nemes
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  5. Mongkol Techakumphu
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  6. Melinda K. Pirity
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  7. Andras Dinnyes
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Correspondence to Andras Dinnyes.

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Rungarunlert, S., Klincumhom, N., Bock, I. et al. Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor. Biotechnol Lett 33, 1565–1573 (2011). https://doi.org/10.1007/s10529-011-0614-8

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  • DOI: https://doi.org/10.1007/s10529-011-0614-8

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