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Thermotolerant and thermostable laccases

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Abstract

Laccases are phenol-oxidizing, usually four-copper containing metalloenzymes. For industrial and biotechnological purposes, laccases were among the first fungal oxidoreductases providing larger-scale applications such as removal of polyphenols in wine and beverages, conversion of toxic compounds and textile dyes in waste waters, and in bleaching and removal of lignin from wood and non-wood fibres. In order to facilitate novel and more efficient bio-catalytic process applications, there is a need for laccases with improved biochemical properties, such as thermostability and thermotolerance. This review gives a current overview on the sources and characteristics of such laccases, with particular emphasis on the fungal enzymes.

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Abbreviations

ABTS, 2:

2-Azinobis(3-ethylbenz-thiazoline-6-sulfonate)

2,6-DMP:

2,6-Dimethoxyphenol

GUA:

Guaiacol (2-methoxyphenol)

MCO:

Multicopper oxidase

SGZ:

Syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehyde azine)

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Acknowledgements

The work was supported by the Academy of Finland (research Grants no 124160, and 205027 and 212303 to K·H., and no 129869 to T.L.). Nina Hakulinen (Department of Chemistry, University of Joensuu, Finland) is thanked for the 3D figure of Melanocarpus albomyces laccase.

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Authors and Affiliations

  1. Department of Applied Chemistry and Microbiology, Viikki Biocenter, University of Helsinki, Helsinki, Finland

    Kristiina Hildén & Taina Lundell

  2. KCL, Espoo, Finland

    Terhi K. Hakala

  3. School of Biology, University of Nottingham, University Park, Nottingham, NG7 2RD, UK

    Kristiina Hildén

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  1. Kristiina Hildén
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  2. Terhi K. Hakala
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  3. Taina Lundell
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Correspondence to Kristiina Hildén.

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Hildén, K., Hakala, T.K. & Lundell, T. Thermotolerant and thermostable laccases. Biotechnol Lett 31, 1117–1128 (2009). https://doi.org/10.1007/s10529-009-9998-0

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