Abstract
The effects of methyl jasmonate, salicylic acid and ethylene on alkaloid accumulation in in vitro cell suspension, hairy roots and rootless shoot cultures of Catharanthus roseus were analyzed. Ajmalicine, but not catharanthine, accumulation was promoted by jasmonate and ethylene treatments in cell suspensions. In hairy roots, jasmonate induced the accumulation of both alkaloids, whereas ethylene only induced catharanthine accumulation. In shoot cultures, positive effects of jasmonate and ethylene were recorded only in vindoline accumulation. Ethylene diminished catharanthine accumulation in these cultures. No effect of salicylic acid was observed in any of the studied in vitro culture systems.
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Abbreviations
- ET:
-
Ethylene
- MeJa:
-
Methyl jasmonate
- SA:
-
Salicylic acid
- TDC:
-
Tryptophan decarboxylase
References
De Luca V, St-Pierre B (2000) The cell and developmental biology of alkaloid biosynthesis. Trends Plant Sci 5:349–364
Hernández-Domínguez E, Vázquez-Flota F (2006) Monoterpenoid alkaloid quantitation by in situ densitometry-thin layer chromatography. J Liq Chromatogr Relat Technol 29:583–590
Hernández-Domínguez E, Campos-Tamayo F, Vázquez-Flota F (2004) Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus. Biotechnol Lett 26:671–674
Islas-Flores I, Loyola-Vargas VM, Miranda-Ham ML (1994) Tryptophan decarboxylase in transformed roots from Catharanthus roseus and its relationship to tryptamine ajmalicine, and catharanthine accumulation during the culture cycle. In vitro Cell Dev Biol 30P:81–83
Rijhwani SK, Shanks JV (1998) Effects of elicitor dosage and exposure time on biosynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol Prog 14:442–449
van der Heijden L, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R (2004) The Catharanthus alkaloids: pharmacology and biotechnology. Curr Med Chem 11:607–628
Vázquez-Flota F, Loyola-Vargas VM (2003) In vitro plant cell culture as the basis for the development of a research institute in Mexico: Centro de Investigación Científica de Yucatán. In vitro Cell DevBiol 39P:250–258
Vázquez-Flota F, Moreno-Valenzuela O, Miranda-Ham ML, Coello-Coello J, Loyola-Vargas VM (1994) Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy roots cultures. Medium optimization and elicitation. Plant Cell Organ Cult 38:273–279
Vázquez-Flota F, Carrillo-Pech M, Minero-García Y, Miranda-Ham ML (2004) Alkaloid metabolism in wounded Catharanthus seedlings. Plant Physiol Biochem 42:623–628
Whitmer S, Canel C, Hallard D, Goncalves C, Verpoorte R (1998) Influence of precursor availability on alkaloid accumulation by transgenic cell line of Catharanthus roseus. Plant Physiol 116:853–857
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Supported by the National Council of Science and Technology (CONCAYT, México).
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10529_2008_9881_MOESM1_ESM.doc
Alkaloid accumulation in C. roseus cell suspension (A), hairy roots (B) and rootless shoots (C) cultures exposed to salicylic acid (1 mM) for the indicated times. Values above the bars corresponding to control indicate the alkaloid content taken as 100%. Average of triplicates, standard deviation was within 9 % of the mean. Seven day-old cell, ten-day old root, and twelve-day old shoot cultures were employed. (DOC 58 kb)
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Vázquez-Flota, F., Hernández-Domínguez, E., de Lourdes Miranda-Ham, M. et al. A differential response to chemical elicitors in Catharanthus roseus in vitro cultures. Biotechnol Lett 31, 591–595 (2009). https://doi.org/10.1007/s10529-008-9881-4
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DOI: https://doi.org/10.1007/s10529-008-9881-4