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Synthesis of glyceryl ferulate by immobilized ferulic acid esterase

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Abstract

Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL “Amano” from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50°C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25°C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.

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Acknowledgment

Pectinase PL “Amano” was supplied by Amano Enzyme Co., Ltd., Nagoya, Japan.

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Authors and Affiliations

  1. Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Takemasa Matsuo, Takashi Kobayashi, Yukitaka Kimura & Shuji Adachi

  2. Research and Development Department, Okumoto Flour Milling Co., Ltd., Kaizuka, Osaka, 597-8515, Japan

    Moriyasu Tsuchiyama & Tadanobu Oh

  3. Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan

    Tatsuji Sakamoto

Authors
  1. Takemasa Matsuo
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  2. Takashi Kobayashi
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  3. Yukitaka Kimura
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  4. Moriyasu Tsuchiyama
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  5. Tadanobu Oh
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  6. Tatsuji Sakamoto
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  7. Shuji Adachi
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Correspondence to Shuji Adachi.

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Matsuo, T., Kobayashi, T., Kimura, Y. et al. Synthesis of glyceryl ferulate by immobilized ferulic acid esterase. Biotechnol Lett 30, 2151–2156 (2008). https://doi.org/10.1007/s10529-008-9814-2

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  • DOI: https://doi.org/10.1007/s10529-008-9814-2

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