Abstract
The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40°C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.
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Kamiya, N., Matsushita, Y., Hanaki, M. et al. Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media. Biotechnol Lett 30, 1037–1040 (2008). https://doi.org/10.1007/s10529-008-9638-0
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DOI: https://doi.org/10.1007/s10529-008-9638-0