Abstract
Pseudomonas stutzeri SDM oxidized dl-lactic acid (25.5 g l-1) into pyruvic acid (22.6 g l-1) over 24 h. Both NAD+-independent d-lactate dehydrogenase and NAD+-independent l-lactate dehydrogenase were found for the first time in the bioconversion of lactate to pyruvate based on the enzyme activity assay and proteomic analysis.
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Acknowledgements
We are grateful to Dr. L. S. Wang at the State Key Laboratory of Microbial Technology in Shandong University for the analytical assistance of MALDI-TOF MS with protein samples.
The work was supported in part by the grant from the State Key Foundation of the Ministry of Education of China (Grant no. 106102).
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Jianrong Hao and Cuiqing Ma contributed equally to this work
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Hao, J., Ma, C., Gao, C. et al. Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate. Biotechnol Lett 29, 105–110 (2007). https://doi.org/10.1007/s10529-006-9204-6
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DOI: https://doi.org/10.1007/s10529-006-9204-6