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Down-regulation of transglutaminase II leads to impaired motility of cancer cells by inactivation of the protein kinase, Akt, and decrease of reactive oxygen species

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Abstract

We employed RNA interference to determine the role of tissue transglutaminase II (TGase II) in motility of cancer cells. Down-regulation of TGase II by small interfering RNA against TGase II impaired adhesion and motility of HeLa cells by decreasing phosphorylation of the protein kinase, Akt, and reactive oxygen species (ROS). Over-expression of TGase II showed opposite effects. These results suggest potential utility of TGase II for development of therapeutic anti cancer vaccine.

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Acknowledgments

This work was supported by a grant from (0103026-1-2) from the Basic Research Program of the Korea Science & Engineering Foundation, a grant from Korea Research Foundation and Vascular System Research Center, a grant from Korea Research Foundation (0805011-1-1), and a grant (A050260) from the Ministry of Health and Welfare of Korea.

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Authors and Affiliations

  1. School of Life Sciences, Kangwon National University, Chunchon, 200-701, Korea

    Jinhee Bae & Dooil Jeoung

  2. Laboratory of Radiation Effect, Division of Radiation Biology, Korea Institute of Radiological and Medical Sciences, Seoul, 139-706, Korea

    Yun-Sil Lee

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  1. Jinhee Bae
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  2. Yun-Sil Lee
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  3. Dooil Jeoung
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Correspondence to Dooil Jeoung.

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Bae, J., Lee, YS. & Jeoung, D. Down-regulation of transglutaminase II leads to impaired motility of cancer cells by inactivation of the protein kinase, Akt, and decrease of reactive oxygen species. Biotechnol Lett 28, 1151–1158 (2006). https://doi.org/10.1007/s10529-006-9079-6

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  • DOI: https://doi.org/10.1007/s10529-006-9079-6

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