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Simultaneous detection of double-stranded RNA-induced protein kinase and its specific mRNA by single cell analysis

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Abstract

Fluorescent in situ hybridization was combined with flow cytometry to detect the expression of the double-stranded-RNA-induced protein kinase (PKR) in single cells. Labeled anti-sense oligonucleotide was used to target the specific mRNA while the protein was targeted with an antibody. It was demonstrated that the PKR-mRNA signal could be protected through a lengthy immunostaining procedure. The expression pattern of the PKR-mRNA with respect to DNA content was shown to be comparable to that of 18S ribosomal RNA.

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Authors and Affiliations

  1. Chemical Engineering Program, University of California La Jolla, California, San Diego, 92093, USA

    Mónica M. Lozano, Tanya L. Chantawansri & Pao C. Chau

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  1. Mónica M. Lozano
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  2. Tanya L. Chantawansri
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  3. Pao C. Chau
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Correspondence to Pao C. Chau.

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Lozano, M.M., Chantawansri, T.L. & Chau, P.C. Simultaneous detection of double-stranded RNA-induced protein kinase and its specific mRNA by single cell analysis. Biotechnol Lett 27, 875–880 (2005). https://doi.org/10.1007/s10529-005-6725-3

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  • DOI: https://doi.org/10.1007/s10529-005-6725-3

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