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Production of Recombinant Human Placental Variant Growth Hormone in Pichia Pastoris

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Abstract

cDNA encoding mature human placental variant growth hormone (HGH-V) was synthesized by retro-transcription polymerase chain reaction (RT-PCR) from total RNA recovered from human term-placenta and cloned in pBluescript plasmid (pBS) in Escherichia coli. cDNA was subcloned into pPIC9, fusing it to the flanking regulatory sequences of the Pichia pastoris alcohol oxidase 1 gene (AOX1) and finally introduced into the genome of this yeast by homologous recombination. The resulting new recombinant strain produced and secreted, towards the culture medium, mature HGH-V, whose activity was demonstrated in cell culture by the Nb2 proliferation assay.

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Authors and Affiliations

  1. Departamento de Bioquímica, Laboratorio de Biotecnología, Facultad de Medicina de la Universidad Autónoma de Nuevo León, Ave. Madero y Dr. Eduardo Aguirre Pequeño, Col. Mitras Centro, 64460, Monterrey, N.L., México

    José Prisco Palma-Nicolás, Jorge A. Ascacio-Martínez, Agnès Revol & Hugo A. Barrera-Saldaña

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  1. José Prisco Palma-Nicolás
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  2. Jorge A. Ascacio-Martínez
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  3. Agnès Revol
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  4. Hugo A. Barrera-Saldaña
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Correspondence to Hugo A. Barrera-Saldaña.

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Palma-Nicolás, J.P., Ascacio-Martínez, J.A., Revol, A. et al. Production of Recombinant Human Placental Variant Growth Hormone in Pichia Pastoris. Biotechnol Lett 27, 1695–1700 (2005). https://doi.org/10.1007/s10529-005-2733-6

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  • DOI: https://doi.org/10.1007/s10529-005-2733-6

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