Abstract
Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. Physical parameters (height and weight) correlated very well with total DNA content (r 2=0.86, re. 0.94). The relation between DNA content and glycosaminoglycan content was less but still significant. No significant relationship was found between the elasticity module and the DNA content, even if the elasticity module increased slightly at higher DNA content. With respect to later implantation, selection of a construct for implantation based on the weight, which can be determined non-invasive and under sterile conditions, seems to be justifiable.
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References
CF Brunk KC Jones TW James (1979) ArticleTitleAssay for nanogram quantities of DNA in cellular homogenates Anal. Biochem. 92 497–500 Occurrence Handle10.1016/0003-2697(79)90690-0 Occurrence Handle1:CAS:528:DyaE1MXpvFCksw%3D%3D
MD Buschmann YA Gluzband AJ Grodzinsky JH Kimura EB Hunziker (1992) ArticleTitleChondrocytes in agarose culture synthesize a mechanically functional extrazellular matrix J. Orthop. Res. 10 745–758 Occurrence Handle1:CAS:528:DyaK3sXlslSmug%3D%3D
RW Farndale DJ Buttle AJ Barrett (1986) ArticleTitleImproved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue Biochim. Biophys. Acta 883 173–177 Occurrence Handle1:CAS:528:DyaL28Xls1yhsrs%3D
R Janßen S Nagel-Heyer CH Goepfert R Pörtner D Toykan O Krummhauer MM Morlock P Adamietz NM Meenen WM Kriven D-K Kim A Tampieri G Celotti (2004) Calcium phosphate ceramics as substrate for cartilage cultivation Ceramic Engineering and Science Proceedings accepted
RK Korhonen MS Laasanen J Töryräs J Rieppo J Hirvonen HJ Helminen JS Jurvelin (2002) ArticleTitleComparison of equilibrium response of articular cartilage in unconfined compression, confined compression and indentation J. Biomech. 35 903–909 Occurrence Handle10.1016/S0021-9290(02)00052-0 Occurrence Handle1:STN:280:DC%2BD38zhtVersA%3D%3D
S Nagel-Heyer R Janßen C Goepfert P Adamietz NM Meenen R Pörtner (2004) ArticleTitleCartilage cultivation on a calcium phosphate ceramic in a bioreactor system Key Eng. Mat. 264–268 2119–2122 Occurrence Handle10.4028/www.scientific.net/KEM.264-268.2119
SS Park MJ Ward (1995) ArticleTitleTissue-engineered cartilage for implantation and grafting Fac. Plast. Surg. 11 278–283 Occurrence Handle10.1055/s-2008-1064544 Occurrence Handle1:STN:280:ByiC1Mflslc%3D
JP Petersen A Rücker Stechow D von P Adamietz R Pörtner JM Rueger NM Meenen (2003) ArticleTitlePresent and future therapies of articular cartilage defects Eur. J. Trauma 1 1–10 Occurrence Handle10.1007/s00068-003-1215-6
M Sittinger O Schultz G Keyser WW Minuth GR Burmester (1997) ArticleTitleArtificial tissues in perfusion culture Int. J. Art. Org. 20 57–62 Occurrence Handle1:STN:280:ByiB3MnmsFU%3D
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Revisions requested14 October 2004; Revisions received 7 December 2004
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Nagel-Heyer, S., Goepfert, C., Morlock, M.M. et al. Relationship between physical, biochemical and biomechanical properties of tissue-engineered cartilage-carrier-constructs. Biotechnol Lett 27, 187–192 (2005). https://doi.org/10.1007/s10529-004-7859-4
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DOI: https://doi.org/10.1007/s10529-004-7859-4