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A Grapevine-Inducible Gene Vv-α-gal/SIP Confers Salt and Desiccation Tolerance in Escherichia coli and Tobacco at Germinative Stage

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Abstract

Grapevine is an important fruit crop cultivated worldwide. Previously, we have reported the characterization of a salt stress-inducible gene Vv-α-gal/SIP isolated from the tolerant grapevine cultivar Razegui. In this study, we performed functional studies in both Escherichia coli and tobacco systems to gain more insights in the role of the Vv-α-gal/SIP gene. Our data revealed that the recombinant E. coli cells harboring the pET24b+ expression vector with the Vv-α-gal/SIP showed higher tolerance to desiccation and salinity compared to E. coli cells harboring the vector alone. In addition, the transgenic tobacco plants expressing the Vv-α-gal/SIP gene exhibited a higher percentage of seed germination and better growth under salt stress than the wild-type (WT) tobacco seedlings. This stress mitigation might be related to the putative function of this gene, which is thought to be involved in carbohydrate metabolism regulation. Collectively, these results suggest that Vv-α-gal/SIP is potentially a candidate gene for engineering drought and salt tolerance in cultivated plants.

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Acknowledgements

This work was funded by the Tunisian–German bilateral project (BMBF/Tunisian, Project Nr. TUNGER-2015-32) and a research grant of the German academic exchange service (DAAD).

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Authors and Affiliations

  1. Laboratory of Plant Molecular Physiology, Biotechnology Center of Borj Cedria, PB.901, 2050, Hammam-Lif, Tunisia

    Samia Daldoul, Anis Ben Amar, Mahmoud Gargouri, Hajer Limam & Ahmed Mliki

  2. DLR Rheinpfalz, Institute of Plant Protection, Breitenweg 71, 67435, Neustadt an Der Weinstraße, Germany

    Samia Daldoul, Anis Ben Amar & Thierry Wetzel

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  1. Samia Daldoul
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  2. Anis Ben Amar
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  3. Mahmoud Gargouri
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Correspondence to Samia Daldoul.

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Daldoul, S., Amar, A.B., Gargouri, M. et al. A Grapevine-Inducible Gene Vv-α-gal/SIP Confers Salt and Desiccation Tolerance in Escherichia coli and Tobacco at Germinative Stage. Biochem Genet 56, 78–92 (2018). https://doi.org/10.1007/s10528-017-9831-8

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