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Prevalence and Molecular Identification of the Mediterranean Variant Among G6PD-Deficient Sistani and Balouch Males in Southeastern Iran

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Abstract

To determine the prevalence of G6PD deficiency and a Mediterranean mutation among males in southeastern Iran, we studied 1,097 Sistani and Balouch schoolboys. A questionnaire was used to collect demographic data and a history of malaria infection; blood samples were evaluated for G6PD deficiency and the G6PD Mediterranean mutation. Of the 1,097 boys screened, 175 were G6PD deficient (5.8 % of the Sistani boys and 19.3 % of the Balouch boys). The malaria survey indicated that among Balouch subjects, malaria infection was about 14 times that of Sistani subjects. Molecular characterization of G6PD-deficient samples revealed a general frequency of 85.1 % for the Mediterranean variant among all subjects (75 % among Sistani and 86.2 % among Balouch cases). The high prevalence of G6PD deficiency among Balouch populations confirms the hypothesis that the distribution of G6PD deficiency is concordant with the geographic distribution of malaria.

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References

  • Al-Allawi N, Eissa AA, Jubrael JMS, Jamal SAR, Hamamy H (2010) Prevalence and molecular characterization of glucose-6-phosphate dehydrogenase deficient variants among the Kurdish population of Northern Iraq. BMC Blood Disord 10(6):1–8

    Google Scholar 

  • Baird J, Hoffman S (2004) Primaquine therapy for malaria. Clin Infect Dis 39:1336–1345

    Article  PubMed  CAS  Google Scholar 

  • Bayoumi RA, Nur-E-Kamal MS, Tadayyon M, Mohamed KK, Mahboob BH, Qureshi MM, Lakani MS, Awaad MO, Kaeda J, Vulliamy TJ, Luzzatto L (1996) Molecular characterization of erythrocyte glucose-6-phosphate dehydrogenase deficiency in Al-Ain district, United Arab Emirates. Hum Hered 46:136–141

    Article  PubMed  CAS  Google Scholar 

  • Beutler E (2008) Glucose-6-phosphate dehydrogenase deficiency: a historical perspective. Blood 111:16–24

    Article  PubMed  CAS  Google Scholar 

  • Beutler E, Mitchell M (1968) Special modifications of the fluorescent screening method for glucose-6-phosphate dehydrogenase deficiency. Blood 32:816–818

    PubMed  CAS  Google Scholar 

  • Beutler E, Vulliamy TJ (2002) Hematologically important mutations: glucose-6-phosphate dehydrogenase. Blood Cells Mol Dis 28:93–103

    Article  PubMed  Google Scholar 

  • Beutler E, Blume KG, Kaplan JC, Löhr GW, Ramot B, Valenline WN (1979) International committee for standardization in hematology: recommended screening test for glucose-6- phosphate dehyrogenase (G6PD) deficiency. Br J Hematol 43(3):456–459

    Article  Google Scholar 

  • Cappellini M, Fiorelli G (2008) Glucose-6-phosphate dehydrogenase deficiency. Lancet 371(9606):64–74

    Article  PubMed  CAS  Google Scholar 

  • Daar S, Vulliamy T (1996) Molecular characterization of G6PD in Oman. Human Hered 49:172–174

    Article  Google Scholar 

  • Edrissian GH (2006) Malaria in Iran: past and present situation. Iranian J Parasitol 1(1):1–14

    Google Scholar 

  • El-Hazmi MAF, Warsy AS (1990) Frequency of G6PD variants and deficiency in Arabia. Gene Georg 4:15–20

    CAS  Google Scholar 

  • Fanello CI, Karema C, Avellino P, Bancone G, Uwimana A, Lee SJ, d’Alessandro U, Modiano D (2008) High risk of severe anaemia after chlorproguanil–dapsone + artesunate antimalarial treatment in patients with G6PD (A−) deficiency. Plos One 3(12):e4031

    Article  PubMed  Google Scholar 

  • Gelpi AP (1965) G6PD deficiency in Saudi Arabia: a survey. Blood 25:486–493

    PubMed  CAS  Google Scholar 

  • Gelpi AP, King MC (1977) New data on glucose-6-phosphate dehydrogenase deficiency in Saudi Arabia: G6PD variants and the association between enzyme deficiency and hemoglobins. Hum Hered 27(4):285–291

    Article  PubMed  CAS  Google Scholar 

  • Karimi M, Martinez di Montemuros F, Danielli MG, Farjadian S, Afrasiabi A, Fiorelli G, Cappellini MD (2003) Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in the Fars province of Iran. Haematologica 88:346–347

    PubMed  Google Scholar 

  • Luzzatto L (1979) Genetics of red cells and susceptibility to malaria. Blood 54:961–976

    PubMed  CAS  Google Scholar 

  • Luzzatto L, Bienzle U (1979) The malaria/G6PD hypothesis. Lancet 1:1183–1184

    Article  PubMed  CAS  Google Scholar 

  • Martinez DI, Montemuros F, Dotti C, Tavazzi D, Fiorelli G, Cappellini M (1997) Molecular heterogeneity of glucose-6-phosphate dehydrogenase (G6PD) variants in Italy. Haematologica 82:440–445

    Google Scholar 

  • Mesbah-Namin SA, Sanati MH, Mowjoodi A, Mason PJ, Vulliamy TJ, Noori-Daloii M (2002) Three major glucose-6-phosphate dehydrogenase-deficient polymorphic variants identified in Mazandaran state of Iran. Br J Hematol 117:763–764

    Article  CAS  Google Scholar 

  • Miri-Moghaddam E, Zadeh-Vakili A, Rouhani Z, Naderi M, Eshghi P, Khazaei Feizabad A (2011) Molecular basis and prenatal diagnosis of beta-thalassemia among Balouch population in Iran. Prenat Diagn 31(8):788–791

    Article  PubMed  CAS  Google Scholar 

  • Missiou T, Sagaraki S (1991) Screening for glucose 6-phosphate dehydrogenase deficiency as a preventive measure: prevalence among 1,286,000 Greek newborn infants. J Pediatr 119:293–299

    Article  Google Scholar 

  • Mohammed AM, Al-Hilli F, Nadkarni KV, Bhagwa GP, Bapat JP (1992) Haemoglobinopathies and G6PD deficiency in hospital birth in Bahrain. Ann Saudi Med 12:536–539

    PubMed  CAS  Google Scholar 

  • Moiz B, Nasir A, Moatter T, Ali Naqvi Z, Khurshid M (2009) Population study of 1311 C/T polymorphism of glucose-6 phosphate dehydrogenase gene in Pakistan: an analysis of 715 X-chromosomes. BMC Genet 10(41):1–7

    Google Scholar 

  • Mortazavi Y, Mirzamohammadi F, Teremahi Ardestani M, Miri-Moghaddam E, Vulliamy TJ (2010) Glucose 6-phosphate dehydrogenase deficiency in Tehran, Zanjan and Sistan-Balouchestan provinces: prevalence and frequency of Mediterranean variant of G6PD. Iran J Biotech 8(4):229–233

    CAS  Google Scholar 

  • Nkhoma E, Poole C, Vannappagari V, Hall S, Beutler E (2009) The global prevalence of glucose-6-phosphate dehydrogenase deficiency: a systematic review and meta-analysis. Blood Cells Mol Dis 42:267–278

    Article  PubMed  CAS  Google Scholar 

  • Noori-Daloii MR, Yousefi A, Mohammad Ganji S, Hejazi SH, Soltani S, Sanei Moghadam E et al (2005) Molecular identification of the most prevalent mutation of glucose-6-phosphate dehydrogenase gene in deficient patients in Sistani and Balochestan province of Iran. J Sci Islam Repub Iran 16(4):321–325

    CAS  Google Scholar 

  • Noori-Daloii MR, Hejazi SH, Yousefi A, Mohammad Ganji S, Soltani S, Javadi KR et al (2006a) Identification of mutations in G6PD gene in patients in Hormozgan Province of Iran. J Sci Islam Repub Iran 17(4):313–316

    CAS  Google Scholar 

  • Noori-Daloii MR, Soltanian S, Mohammad Gangi SH, Yousefi A, Hejazi S, Bani-hashem A et al (2006b) Molecular identification of the most prevalent mutations of glucose-6-posphate dehydrogenase (G6PD) gene in deficient patients in Khorasan province of Iran. J Sci Islam Repub Iran 17(2):103–106

    CAS  Google Scholar 

  • Oner R, Gümrük F, Acar C, Oner C, Gürgey A, Altay C (2000) Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in Turkey. Haematologica 85:320–321

    PubMed  CAS  Google Scholar 

  • Oppenheim A, Jury CL, Rund D, Vulliamy TJ, Luzzatto L (1993) G6PD Mediterranean accounts for the high prevalence of G6PD deficiency in Kurdish Jews. Hum Genet 91:293–294

    Article  PubMed  CAS  Google Scholar 

  • Rahimi Z, Vaisi-Raygani A, Nagel RL, Muniz A (2006) Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in the Kurdish population of western Iran. Blood Cells Mol Dis 37:31–37

    Article  Google Scholar 

  • Ruwende C, Hill A (1998) Glucose-6-phosphate dehydrogenase deficiency and malaria. J Mol Med 76:581–588

    Article  PubMed  CAS  Google Scholar 

  • Segeja MD, Mmbando BP, Kamugisha ML, Akida JA, Savaeli ZX, Minja DT, Msangeni HA, Lemnge MM (2008) Prevalence of glucose-6-phosphate dehydrogenase deficiency and haemoglobin S in high and moderate malaria transmission areas of Muheza, north-eastern Tanzania. Tanzan J Health Res 10(1):9–13

    PubMed  CAS  Google Scholar 

  • Shaker Y, Onsi A, Aziz R (1966) The frequency of G6PD deficiency in the newborns and adults in Kuwait. Am J Hum Genet 18:609–613

    PubMed  CAS  Google Scholar 

  • Theerathananon W, Francois JJ, Zongram O, Pumpaibool T, Hounnaklang N, Seugorn A, Siriporn N, Harnyuttanakorn P, Kanchanakhan N (2010) Prevalence of G6PD deficiency in malaria endemic area: case study in Bongti sub-district, Saiyok district, Kanchanaburi province, Thailand. J Health Res 24(1):55–62

    Google Scholar 

  • Vulliamy TJ, D’Urso M, Battistuzzi G, Estrada M, Foulkes NS, Martini G, Calabro V, Poggi V, Giordano R, Town M (1988) Diverse point mutations in the human glucose-6-phosphate dehydrogenase gene cause enzyme deficiency and mild or severe hemolytic anemia. Proc Natl Acad Sci USA 85(14):5171–5175

    Article  PubMed  CAS  Google Scholar 

  • White JM, Christi BS, Nam D, Daar S, Higgs DR (1993) Frequency and clinical significance of erythrocyte genetic abnormalities in Omanis. J Med Genet 30:396–400

    Article  PubMed  CAS  Google Scholar 

  • WHO Working Group (1989) Glucose-6-phosphate dehydrogenase deficiency. Bull World Health Organ 67:601–611

    Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Deputy of Research in Zahedan University of Medical Sciences for financial support for this study and education offices in the regions where the study was carried out. We also thank all student subjects who kindly participated in this study.

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Correspondence to Ebrahim Miri-Moghaddam.

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Miri-Moghaddam, E., Mortazavi, Y., Nakhaee, A. et al. Prevalence and Molecular Identification of the Mediterranean Variant Among G6PD-Deficient Sistani and Balouch Males in Southeastern Iran. Biochem Genet 51, 131–138 (2013). https://doi.org/10.1007/s10528-012-9548-7

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  • DOI: https://doi.org/10.1007/s10528-012-9548-7

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