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Investigation of the Relationship Between Oxidative Stress and Glucose Signaling in Schizosaccharomyces pombe

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Abstract

The invertase mutant defective in the glucose signaling pathway of Schizosaccharomyces pombe (ird11) is resistant to glucose repression. This mutant is able to consume sucrose alongside glucose and grows in glucose-containing media with a generation time close to that of the wild type. Intracellular oxidation, protein carbonyl, and reduced glutathione levels and catalase, superoxide dismutase, and glutathione peroxidase activity were investigated in ird11, to determine the relationship between oxidative stress response and glucose signaling. The expression profiles of some genes involved in regulation of glucose repression (fbp1, fructose-1,6-bis-phosphatase; hxk2, hexokinase) and stress response (atf1 and pap1 transcription factors; ctt1, catalase; sod1, Cu,Zn superoxide dismutase) were analyzed using the quantitative real-time PCR technique. Oxidative stress response in ird11 seems to be affected by glucose signaling in a manner different from that caused by glucose deprivation.

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Acknowledgments

This research was supported by Istanbul University Research Fund, Projects BAP1477 and UDP-4218.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, 34134, Istanbul, Turkey

    Bedia Palabiyik, Cenk Kig, Murat Pekmez, Levent Dalyan, Nazli Arda & Guler Temizkan

  2. Department of Molecular Cell Biology, Catholic University of Leuven, 3000, Leuven, Belgium

    Cenk Kig

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  1. Bedia Palabiyik
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Correspondence to Bedia Palabiyik.

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Palabiyik, B., Kig, C., Pekmez, M. et al. Investigation of the Relationship Between Oxidative Stress and Glucose Signaling in Schizosaccharomyces pombe . Biochem Genet 50, 336–349 (2012). https://doi.org/10.1007/s10528-011-9477-x

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  • DOI: https://doi.org/10.1007/s10528-011-9477-x

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