Skip to main content
Log in

Assessment of Genetic Relatedness of Crossbred Chicken Populations Using Microsatellite Markers

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

To measure genetic relatedness between populations, for breeding purposes, we analyzed 170 birds from six crossbred populations of three pure lines of White Leghorn chickens, using 14 microsatellite markers. All the microsatellites were polymorphic, with 2–6 alleles. The mean number of alleles per locus was 3.21. The effective number of alleles varied from 1.14 to 3.94. The observed heterozygosity varied from 0.133 to 1.00, with a mean of 0.748. The F IS values were mostly negative, with an average of −0.345. The mean F ST value was 0.056. The Nm values ranged from 1.91 to 42.17. The highest genetic identity was observed between IWI × IWK and IWK × IWI. The relation between any two groups of crosses was more than 85%. The results suggest that the crossbred populations were very closely related.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Bhattacharya TK, Rani S, Maiti SK, Dayal S, Kumar P, Sharma A (2007) Polymorphism of ZuBeCa3 microsatellite and its association with mammary tumor in dogs. Int J Immunogenet 33:161–165

    Article  Google Scholar 

  • Cheng HH, Levin I, Vallejo RL, Khatib H, Dodgson JB, Crittenden LB, Hillel J (1995) Development of a genetic map of the chicken with markers of high utility. Poult Sci 74:1855–1874

    CAS  PubMed  Google Scholar 

  • Crooijmans RPMA, Groen AF, Van Kampen AJA, van der Beek S, van der Poel JJ, Groenen MAM (1996) Microsatellite polymorphism in commercial broiler and layer lines estimated using pooled blood samples. Poult Sci 75:904–909

    CAS  PubMed  Google Scholar 

  • Flock D, Preisinger R (2002) Breeding plan for poultry with emphasis on sustainability. In: Seventh World Conference on Genetic Applications for Livestock Production, 19–23 August, Montpelliers, France

  • Hillel J, Groenen AM, Biochard MT, Korol AB, David L, Kirzhner VM, Burke T, Dirie AB, Crooijmans RPMA, Elo K, Feldman MW, Freidlin PJ, Tanila AM, Oortwijn M, Thomson P, Vignal A, Wimmers K, Weigend S (2003) Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools. Genet Sel Evol 35:533–557

    Article  CAS  PubMed  Google Scholar 

  • Nei M (1972) Genetic distance between populations. Am Nat 106:283–292

    Article  Google Scholar 

  • Nevo E (1978) Genetic variation in natural populations: patterns and theory. Theor Popul Biol 13:21–177

    Article  Google Scholar 

  • Olowofeso O, Wang JY, Dail GJ, Yang Y, Mekki DM, Musa HH (2005) Measurement of genetic parameters within and between Haimen chicken populations using microsatellite markers. Int J Poult Sci 4:143–148

    Article  Google Scholar 

  • Osman SA, Sekino M, Nishibori M, Kawamoto Y, Kinoshita K, Yamamoto Y, Tsudzuki M (2004) Genetic variability and relationships of Japanese native chickens assessed by means of microsatellite profiling approach, focussing on the Oh-Shamo (Japanese large game) and its related breeds. J Poult Sci 41:94–109

    Article  CAS  Google Scholar 

  • Pandey AK, Kumar Dinesh, Sharma R, Sharma U, Vijh RK, Ahlawat SPS (2005) Population structure and genetic bottleneck analysis of Ankleshwar poultry breed by microsatellite markers. Asian-Aust J Anim Sci 18:915–921

    CAS  Google Scholar 

  • Pirany N, Romanov MN, Ganpule SP, Devegowda G, Prasad DT (2007) Microsatellite analysis of genetic diversity in Indian chicken populations. J Poult Sci 44:19–28

    Article  CAS  Google Scholar 

  • Rajkumar U, Gupta BR, Reddy AR (2008) Genomic heterogeneity of chicken populations in India. Asian, Aust J Anim Sci

    Google Scholar 

  • Romanov MN, Weigend S (2001) Analysis of genetic relationships between various populations of domestic and Red Jungle fowl using microsatellite markers. Poult Sci 80:1057–1063

    CAS  PubMed  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, vol 1, 3rd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Takahashi H, Nakamura A (2007) Genetic diversity and differentiation of the Nagoya breed inferred from microsatellite DNA polymorphisms. J Poult Sci 44:135–140

    Article  CAS  Google Scholar 

  • Wimmers K, Ponsuksili S, Hardge T, Valle-Zarate A, Mathur PK, Horst P (2000) Genetic distances of African, Asian and South American local chickens. Anim Genet 31:159–165

    Article  CAS  PubMed  Google Scholar 

  • Yu YB, Wang JY, Mekki DM, Tang QP, Li HF (2006) Evaluation of genetic diversity and genetic distance between twelve Chinese indigenous chicken breeds based on microsatellite markers. Int J Poult Sci 5:550–556

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Rudra Nath Chatterjee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chatterjee, R.N., Bhattacharya, T.K., Dange, M. et al. Assessment of Genetic Relatedness of Crossbred Chicken Populations Using Microsatellite Markers. Biochem Genet 48, 727–736 (2010). https://doi.org/10.1007/s10528-010-9355-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-010-9355-y

Keywords

Navigation