Skip to main content
SpringerLink
Log in

Effect of Trichoderma spp. isolates for biological control of tan spot of wheat caused by Pyrenophora tritici-repentis under field conditions in Argentina

  • Original Research Paper
  • Published:
BioControl Aims and scope Submit manuscript

Abstract

Trichoderma spp. have been used as biocontrol agents to protect plants against foliar diseases in several crops, but information from field assays is scarce. In the present work, experiments were carried out to determine the effect of six isolates of Trichoderma harzianum and one isolate of T. koningii on the incidence and severity of tan spot, caused by Pyrenophora tritici-repentis (anamorph: Drechslera tritici-repentis) under field conditions. Significant differences between years, wheat cultivars and treatments were found. In 2003, two of the isolates assayed (T5, T7) showed the best performance against the disease applied as seed treatments or sprayed onto wheat leaves at different stages. The application of six of the treatments on wheat plants significantly reduced disease severity by 16 to 35% in comparison with the control. Disease control provided by isolate T7 was similar to that provided by the fungicide treatment (56% reduction). This is the first report on the efficacy of Trichoderma spp. against tan spot under field conditions in Argentina.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alippi AM, Perelló AE, Sisterna MN, Greco N, Cordo C (2000) Potential of spore-forming bacteria as biocontrol agents of wheat foliar diseases under laboratory and greenhouse conditions. Z Pflanzankr Pflanzanschutz 107:155–169

    Google Scholar 

  • Annone JG (1997) Tan spot of wheat in Argentina: importance and disease management strategies. Helminthosporium blights of wheat. Spot Blotch and Tan Spot. Proceedings of an International Workshop Held at CIMMYT. El Batán, México, pp 339

    Google Scholar 

  • Annone JG (2005) El desafío del manejo integrado de enfermedades de los cultivos en sistemas agrícolas conservacionistas: El caso de la sanidad del trigo en siembra directa. In: Proceedings of the 13th Congreso Latinoamericano de Fitopatología, Villa Carlos Paz, Córdoba. 19–22 de abril de 2005, pp 161–164 (640 pp)

  • Cook RJ, Baker J (1974) In: Cook RJ 1993. Making greater use of introduced microorganisms for biological control of plant pathogens. Annu Rev Phytopathol 31:53–80

    Google Scholar 

  • Cook RJ, Veseth J (1991) Wheat health management. Plant Health Series. American Phytopathology Society Press, 152 pp

  • Cordo C, Mónaco C, Segarra C, Simón MR, Bayo D, Mansilla A, Kripels N, Conde R, Perello A (2007) Trichoderma spp. as elicitor of wheat plant defense responses against Septoria tritici. Biocontrol Sci Technol (in press)

  • Dal Bello G, Mónaco C, Simón MR (2002) Biological control of seedling blight of wheat caused by Fusarium graminearum with beneficial rhizosphere microorganisms. World J Microbiol Biotechnol 18:627–636

    Article  Google Scholar 

  • Elad Y (2000) Biological control of foliar pathogens by means of T. harzianum and potential modes of action. Crop Prot 19:709:714

    Article  Google Scholar 

  • Harman G (2000) Myths and dogmas of biocontrol. Changes in perceptions derived from research on Trichoderma harzianum T-22. Plant Dis 84:377–393

    Article  Google Scholar 

  • Harman GE (2004) Overview of new insights into mechanisms and uses of Trichoderma based products. Phytopathology 94:S138

    Article  Google Scholar 

  • Hermosa MR, Grondona I, Iturriaga EA, Díaz-Miguez JM, Castro C, Monte E, Garcia-Acha I (2000) Molecular characterization and identification of biocontrol isolates of Trichoderma spp. Appl Environ Microbiol 66:1890–1898

    Article  PubMed  CAS  Google Scholar 

  • Lee S, Gough J (1984) Inheritance of Septoria leaf blotch (Septoria tritici) and Pyrenophora tan spot (Pyrenophora tritici-repentis) resistance in Triticum aestivum cv. Carifen 12. Plant Dis 68:848–851

    Article  Google Scholar 

  • Li B, Sutton J (1995) Evaluation of leaf associated microorganisms for biocontrol of tan spot in wheat foliage. Fitopatol Brasil 20:545–552

    Google Scholar 

  • Lorito M, Woo SL (2004) The molecular biology of the interaction between Trichoderma, phytopathogenic fungi and plants. Phytopathology 94:S139

    Google Scholar 

  • Luz WC (1992) Seed microbiolization of wheat to control Drechslera tritici-repentis. Proceedings of the Second International Tan Spot Workshop Fargo, North Dakota State University, pp 67

  • Melo Y (1991) Potencialidades de utilizacao de Trichoderma spp. no controle biológico de doencas de plantas. In: Controle biológico de doencas de plantas, Chapter 9. EMBRAPA, Brasilia, DF, pp 135–156 (388 p)

  • Mónaco C (1989) Ensayos in-vitro para evaluar la eficiencia de microparásitos sobre esclerocios de Sclerotinia sclerotiorum (Lib) de Bary con miras a su control. Rev Facult Agron 65:76–73

    Google Scholar 

  • Mónaco C, Rollán C, Nico A (1998) Efecto de micoparásitos sobre la capacidad reproductiva de Sclerotinia sclerotiorum. Rev Iberoam Micol 15:84–87

    Google Scholar 

  • Mónaco C, Sisterna MN, Perelló A, DalBello G (2004) Preliminary studies on biological control of the blackpoint complex of wheat in Argentina. World J Microbiol Biotechnol 20:285–290

    Google Scholar 

  • Monte E (2001) Underestanding Trichoderma: between biotechnology and microbial ecology. Int Microbiol 4:1–4

    PubMed  CAS  Google Scholar 

  • Papavizas GC (1985) Trichoderma and Gliocladium: biology, ecology and potencial for biocontrol. Annu Rev Phytopathol 23:23–54.

    Article  Google Scholar 

  • Paulitz TC, Matta A (2000) IV (B) Biological Control of Diseases. 28. The role of the host in biological control of diseases, pp 394–410 In: Albajes R, Gullino ML, van Lenteren JC, Elad Y (eds) Integrated pest and disease management in greenhouse crops. Series: Developments in plant Pathology, vol 14. Kluwer Academic Publisher, Wageningen, The Netherlands, 568 p

  • Perelló AE, Cordo CA, Arriaga HO, Alippi HE (1991) Variation in virulence of Septoria tritici Rob. Ex Desm isolates in wheat. Agronomie 11:571–579

    Article  Google Scholar 

  • Perelló A, Mónaco C, Cordo C (1997) Evaluation of Trichoderma harzianum and Gliocladium roseum in controlling leaf blotch of wheat (Septoria tritici) under in vitro and greenhouse conditions. Z Pflanzenkr Pflanzenschutz 104:588–598

    Google Scholar 

  • Perelló A, Simón MR, Arambarri AM, Cordo C (2001a) Greenhouse screening of the saprophytic resident microflora for control of leaf spots of wheat (Triticum aestivum L). Phytoparasitica 29:341–351

    Google Scholar 

  • Perelló A, Simón MR, Sisterna M, Cordo C, Arambarri AM (2001b) Microflora of wheat (Triticum aestivum L.) in Buenos Aires Province (Argentina) and its possible significance in biological control of foliar pathogens. Z Pflanzankr Pflanzanschutz 108:459–471

    Google Scholar 

  • Perelló A, Simón MR, Arambarri AM (2002) Interactions between foliar pathogens and the saprophytic mycoflora of the wheat (Triticum aestivum L.) phylloplane. J Phytopathol 150:232–243

    Article  Google Scholar 

  • Perelló A, Mónaco C, Sisterna M, Dal Bello G (2003) Biocontrol efficacy of Trichoderma isolates for Tan Spot of wheat in Argentina. Crop Prot 22:1099–1106

    Article  Google Scholar 

  • Perelló A, Mónaco C, Moreno MV, Cordo C, Simón MR (2006) The effect of Trichoderma harzianum and T. koningii on the control of tan spot (Pyrenophora tritici-repentis) and leaf blotch (Mycosphaerella graminicola) of wheat under field conditions in Argentina. Biocontrol Sci Technol 16(8):803–813

    Google Scholar 

  • Pfender WF (1988) Supression os ascocarp formation in Pyrenophora tritici-repentis by Lymonomyces roseipellis, a basidiomycte from reduce-tillage wheat straw. Phytopathology 78:1254–1258

    Article  Google Scholar 

  • Pfender WF, Zhang W, Nus A (1989) Field perfomance and greenhouse assay of fungi for biocontrol of residue borne Pyrenophora tritici-repentis. Phytopathology 79:1152

    Google Scholar 

  • Platt H, Morral RA, Gruen HE (1977) The effects of substrate, temperature, and photoperiod on conidiation of Pyrenophora tritici-repentis. Can J Bot 55:254–259

    Article  Google Scholar 

  • Powell K (1993) Is biocontrol the answer for sustainable agriculture? Mycologist 7:75–78

    Google Scholar 

  • Raymond PJ, Bockus WW (1982) An in vitro technique for profuse sporulation of Drechslera tritici-repentis. Phytopathology 72:934

    Google Scholar 

  • Sutton JC (2005) Present and future perspectives of biological disease control in crops. Proceedings of the 13th Congreso Latinoamericano de Fitopatología; 2005 April 19–22. Villa Carlos Paz, Córdoba, Argentina, pp 640

    Google Scholar 

  • Tronsmo A (1986) Use of Trichoderma spp. in biological control of necrotrophic pathogens. In: Fokkema N, Der Heuvel J (eds) Microbiology of the phyllosphere. Cambridge University Press, Cambridge

    Google Scholar 

  • Windels CE, Lindows SE (1985) Biological control on the phylloplane. American Phytopathology Society Press, St. Paul, MN, pp 169

    Google Scholar 

  • Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421

    Article  Google Scholar 

Download references

Acknowledgements

This work was funded by the Universidad Nacional de La Plata, Project A142. The authors thank the staff of the Estación Experimental J. Hirschhorn, P. Gannon and S. Rapetti, for their technical assistance, and Dr Richard Weinzierl, Professor and Extension Entomologist of the Department of Crop Sciences, University of Illinois, Urbana, for the English checked.

Author information

Authors and Affiliations

  1. Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Calle 60 y 119, La Plata, Provincia de Buenos Aires, CP 1900, Argentina

    Analía Perelló, Virginia Moreno & Cecilia Mónaco

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), La Plata, Argentina

    Analía Perelló & Virginia Moreno

  3. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), La Plata, Argentina

    Cecilia Mónaco

  4. Cerealicultura, Universidad Nacional de La Plata, La Plata, Argentina

    María Rosa Simón

Authors
  1. Analía Perelló
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Virginia Moreno
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cecilia Mónaco
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. María Rosa Simón
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Analía Perelló.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perelló, A., Moreno, V., Mónaco, C. et al. Effect of Trichoderma spp. isolates for biological control of tan spot of wheat caused by Pyrenophora tritici-repentis under field conditions in Argentina. BioControl 53, 895–904 (2008). https://doi.org/10.1007/s10526-007-9110-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10526-007-9110-4

Keywords

Search

Navigation