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Regenerative Potential of Granulocyte Colony-Stimulating Factor Immobilized by Using Electron-Beam Synthesis Nanotechnology in an Experimental Model of Ovarian Reserve Depletion

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Bulletin of Experimental Biology and Medicine Aims and scope

The pharmacological activity of granulocyte CSF (G-CSF) immobilized using electron-beam synthesis nanotechnology (imG-CSF) was evaluated in an experimental model of ovarian reserve depletion. The effectiveness of the drug was compared with that of its unmodified form. Depletion of the ovarian follicular pool in female Sprague-Dawley rats was caused by a single intravenous injection of the antitumor drug etoposide in the maximum tolerated dose. The effectiveness of the studied drugs was assessed by serum concentration of anti-Mullerian hormone (AMH) measured by ELISA and by the number of primordial, two-layer, multilayer, and atretic follicles counted on serial sections of the ovaries (5-μm thick; through the entire organ) stained with hematoxylin and eosin. It was found that imG-CSF prevents depletion of the ovarian reserve in the model used, which was confirmed by high AMH concentration and higher numbers of primordial, two- and multilayer follicles in comparison with the corresponding parameters in the control (etoposide), and by a decrease in the severity of atretic processes. Unmodified form of the drug demonstrated lower efficiency.

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Authors and Affiliations

  1. E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

    T. G. Borovskaya, A. V. Vychuzhanina, A. A. Ligacheva, Yu.A. Shchemerova, L. A. Sandrikina & A. M. Dygai

  2. Research Institute of Clinical and Experimental Lymphology — Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    P. G. Madonov & F. A. Rakitin

  3. Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

    V. E. Goldberg

Authors
  1. T. G. Borovskaya
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  2. A. V. Vychuzhanina
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  3. A. A. Ligacheva
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  4. Yu.A. Shchemerova
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  5. L. A. Sandrikina
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  6. P. G. Madonov
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  7. F. A. Rakitin
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  8. V. E. Goldberg
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  9. A. M. Dygai
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Corresponding author

Correspondence to T. G. Borovskaya.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 176, No. 7, pp. 117-121, July, 2023

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Borovskaya, T.G., Vychuzhanina, A.V., Ligacheva, A.A. et al. Regenerative Potential of Granulocyte Colony-Stimulating Factor Immobilized by Using Electron-Beam Synthesis Nanotechnology in an Experimental Model of Ovarian Reserve Depletion. Bull Exp Biol Med 176, 101–104 (2023). https://doi.org/10.1007/s10517-023-05975-z

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  • DOI: https://doi.org/10.1007/s10517-023-05975-z

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