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The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer

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Bulletin of Experimental Biology and Medicine Aims and scope

We performed a comparative quantitative analysis of LINE-1 mRNA levels in extracellular total plasma RNA in patients with colon cancer and practically healthy donors. Quantitative multiplex PCR with reverse transcription was used to assess the level of LINE-1 and 18S rRNA mRNA in extracellular total plasma RNA. The median of LINE-1 mRNA values in colon cancer patients (4.95) was significantly higher than in healthy donors (2.3) (p=0.037). It was shown for the first time that the level of LINE-1 mRNA in total RNA from blood plasma can be determined in the format of a liquid biopsy and serve as a new potential non-invasive marker of colon cancer.

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    M. L. Filipenko, U. A. Boyarskikh, K. V. Subbotina & E. A. Khrapov

  2. N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia

    I. S. Stilidi & N. E. Kushlinskii

  3. City Clinical Hospital No. 1, Novosibirsk, Russia

    A. V. Sokolov

  4. M. F. Vladimirsky Moscow Regional Research Clinical Institute, Moscow, Russia

    L. S. Leskov

Authors
  1. M. L. Filipenko
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  2. U. A. Boyarskikh
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  3. L. S. Leskov
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  4. K. V. Subbotina
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  5. E. A. Khrapov
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  6. A. V. Sokolov
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  7. I. S. Stilidi
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  8. N. E. Kushlinskii
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Correspondence to M. L. Filipenko.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 2, pp. 242-245, February, 2022

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Filipenko, M.L., Boyarskikh, U.A., Leskov, L.S. et al. The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer. Bull Exp Biol Med 173, 261–264 (2022). https://doi.org/10.1007/s10517-022-05530-2

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  • DOI: https://doi.org/10.1007/s10517-022-05530-2

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