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Effect of Nitric Oxide on the Functioning of the P-Glycoprotein Transporter

  • BIOPHYSICS AND BIOCHEMISTRY
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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of nitric oxide (NO) on the functioning of P-glycoprotein transporter (Pgp) in Caco-2 cells. NO donor S-nitrosoglutathione (GSNO) was used in concentrations of 1, 10, 50, 100, and 500 μM; the duration of exposure was 24 h. The content of Pgp was analyzed by the Western blotting, activity of the transport protein was analyzed by the transport of its substrate fexofenadine. It was shown that GSNO in concentrations of 10 and 50 μM increased the content and activity of Pgp. Increasing the GSNO concentration to 500 μM led to the development of nitrosative stress and a decrease in the content and activity of the transporter protein.

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Authors and Affiliations

  1. Ryazan State Medical University, Ministry of Health of the Russian Federation, Ryazan, Russia

    Yu. V. Abalenikhina, E. A. Sudakova, A. A. Slepnev, A. V. Shchul’kin & E. N. Yakusheva

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  1. Yu. V. Abalenikhina
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  2. E. A. Sudakova
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  3. A. A. Slepnev
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  4. A. V. Shchul’kin
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  5. E. N. Yakusheva
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Correspondence to Yu. V. Abalenikhina.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 1, pp. 38-42, January, 2022

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Abalenikhina, Y.V., Sudakova, E.A., Slepnev, A.A. et al. Effect of Nitric Oxide on the Functioning of the P-Glycoprotein Transporter. Bull Exp Biol Med 173, 28–32 (2022). https://doi.org/10.1007/s10517-022-05486-3

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  • DOI: https://doi.org/10.1007/s10517-022-05486-3

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