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Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin

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The study examined maturation of preovulatory germinal vesicles oocytes (GV oocytes) induced by gonadotropic hormone PMSG in the inbred C57Bl/6J mice (viewed as a gold standard for diverse biomedical studies) as well as in the first generation hybrid C57Bl/6J×СВА/lac and СВА/lac×C57Bl/6J mice at various ages. The most effective donors of GV oocytes were СВА/lac×C57Bl/6J mice (F1 hybrids) yielding 25±2 oocyte/mouse. In contrast, a significantly smaller number of GV oocytes can be isolated from the ovaries of female C57Bl/6J or C57Bl/6J×СВА/lac mice under the same conditions. At this, the greatest number of GV oocytes (42±4) can be retrieved from the ovaries of immature hybrid СВА/lac×C57Bl/6J mice aged 4 weeks. These mice demonstrated the largest share of GV oocytes, which attained MII stage during in vitro culturing. The data conclude that F1 hybrid СВА/lac×C57Bl/6J mice can be viewed as a handy experimental source yielding a large number of GV oocytes capable of meiotic maturation in a culture.

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Authors and Affiliations

  1. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    Ya. A. Riabukha, D. A. Petrova & O. V. Zatsepina

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  1. Ya. A. Riabukha
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  2. D. A. Petrova
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  3. O. V. Zatsepina
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Correspondence to O. V. Zatsepina.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 5, pp. 650-654, May, 2019

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Riabukha, Y.A., Petrova, D.A. & Zatsepina, O.V. Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin. Bull Exp Biol Med 167, 702–705 (2019). https://doi.org/10.1007/s10517-019-04603-z

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  • DOI: https://doi.org/10.1007/s10517-019-04603-z

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