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Endothelial Cells Modulate Differentiation Potential and Mobility of Mesenchymal Stromal Cells

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We studied the effect of endothelial cells on in vitro migration and differentiation potential of multipotent mesenchymal stromal cells. Down-regulation of stemness genes OCT4, SOX2, and chondrogenic differentiation regulator SOX9 gene and upregulation of osteogenesis master-gene RUNX2 in mesenchymal stromal cells were observed in the presence of intact and TNFα-activated endothelial cells, which indicated an increase in commitment of mesenchymal stromal cells.The medium conditioned by endothelial cells stimulated migration activity of mesenchymal stromal cells; migration rate increased significantly in conditioned medium from activated cells in comparison with medium from non-activated cells. It was concluded that the interaction with endothelial cells modulated functional activity of mesenchymal stromal cells; moreover, activated endothelial cells produced more pronounced effects on differentiation potential and migration activity of mesenchymal stromal cells both in direct contact and through paracrine regulation.

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Authors and Affiliations

  1. State Scientific Center Institute of Biomedical Problems, the Russian Academy of Sciences, Moscow, Russia

    O. V. Zhidkova, E. R. Andreeva & L. B. Buravkova

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  1. O. V. Zhidkova
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  2. E. R. Andreeva
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  3. L. B. Buravkova
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Correspondence to O. V. Zhidkova.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 15-19, January, 2018

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Zhidkova, O.V., Andreeva, E.R. & Buravkova, L.B. Endothelial Cells Modulate Differentiation Potential and Mobility of Mesenchymal Stromal Cells. Bull Exp Biol Med 165, 127–131 (2018). https://doi.org/10.1007/s10517-018-4113-y

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  • DOI: https://doi.org/10.1007/s10517-018-4113-y

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