We studied the effect of lipoxygenase inhibitors nordihydroguaiaretic acid (NDGA) and fungus Lecanicillum lecanii extract on lymphatic leukemia P388 cells. The cells grown in the abdominal cavity of DBA2 mice for 7 days were transferred into a nutrient medium. The effect of lipoxygenase inhibitors was evaluated by changes in cell number, trypan blue staining, nucleus damage, and changes in cell distribution by DNA content after 22-h incubation. NDGA and fungus extract induced apoptotic death of lymphatic leukemia cells, which was seen from nucleus damage and reduced DNA content in cells. IC50 for NDGA and fungus extract was 0.66 and 5.5 μg/ml, respectively.
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Bibikova MV, Spiridonova IA, Danilenko AN, Kim YuA, Korystova AF, Shaposhnikova VV, Korystov YuN. Screening of organisms producing inhibitors of 15-lipoxygenase among micromycetes. Antibiotiki Khimioterapiya. 2016;61(7-8):3-9. Russian.
Da Silva EL, Tsushida T, Terao J. Inhibition of mammalian 15-lipoxygenase-dependent lipid peroxidation in low-density lipoprotein by quercetin and quercetin monoglucosides. Arch. Biochem. Biophys. 1998;349(2):313-320.
Hermann M, Kapiotis S, Hofbauer R, Exner M, Seelos C, Held I, Gmeiner B. Salicylate inhibits LDL oxidation initiated by superoxide/nitric oxide radicals. FEBS Lett. 1999;445(1):212-214.
Hodgson JM, Croft KD, Puddey IB, Mori TA, Beilin LJ. Soybean isoflavonoids and their metabolic products inhibit in vitro lipoprotein oxidation in serum. J. Nutr. Biochem. 1996;7(12):664-669.
Hofmanova J, Musilova E, Kozubik A. Suppression of human cancer cell proliferation by lipoxygenase inhibitors and gamma-radiation in vitro. Gen. Physiol. Biophys. 1996;15(4):317-331.
Kopp E, Ghosh S. Inhibition of NF-kappa B by sodium salicylate and aspirin. Science 1994;265:956-959.
Korystov YN, Dobrovinskaya OR, Shaposhnikova VV, Eidus LKh. Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation. FEBS Lett. 1996;388(2-3):238-241.
Michell RH. Inositol lipids in cellular signalling mechanisms. Trends Biochem. Sci. 1992;17(8):274-276.
Nishizuka Y. Protein kinase C and lipid signaling for sustained cellular responses. FASEB J. 1995;9(7):484-496.
Sagone AL Jr, Husney RM. Oxidation of salicylates by stimulated granulocytes: evidence that these drugs act as free radical scavengers in biological systems. J. Immunol. 1987;138(7):2177-2183.
Shaposhnikova VV, Dobrovinskaya OR, Eidus LKh, Korystov YN. Dependence of thymocyte apoptosis on protein kinase C and phospholipase A2. FEBS Lett. 1994;348(3):317-319.
Shaposhnikova VV, Kublik LN, Narimanov AA, Levitman MKh, Orlova OE, Kudryavtsev AA, Leshchenko VV, Korystov YuN. Growth inhibition and death induction in tumor cells by inhibitors of phospholipase A2 and lipoxygenase. Biol. Bull. 2001;28(2):202-205.
Sparrow CP, Parthasarathy S, Steinberg D. Enzymatic modification of low density lipoprotein by purified lipoxygenase plus phospholipase A2 mimics cell-mediated oxidative modification. J. Lipid. Res. 1988;29:745-753.
Tang DG, Honn KV. Apoptosis of W256 carcinosarcoma cells of the monocytoid origin induced by NDGA involves lipid peroxidation and depletion of GSH: role of 12-lipoxygenase in regulating tumor cell survival. J. Cell. Physiol. 1997;172(2):155-170.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 3, pp. 309-312, March, 2017
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Bibikova, M.V., Spiridonova, N.A., Korystova, A.F. et al. Lipoxygenase Inhibitors Nordihydroguaiaretic Acid and Fungus Lecanicillum lecanii Extract Induce Death of Lymphoid Leukemia Cells. Bull Exp Biol Med 163, 330–333 (2017). https://doi.org/10.1007/s10517-017-3796-9
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DOI: https://doi.org/10.1007/s10517-017-3796-9